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Anti-PCYT1A Antibody (4E85) 是一种 Rabbit 抗体,靶向 PCYT1A。Anti-PCYT1A Antibody (4E85) 可用于 WB,ICC/IF,IP,FCM。
Anti-PCYT1A Antibody (4E85) 是一种 Rabbit 抗体,靶向 PCYT1A。Anti-PCYT1A Antibody (4E85) 可用于 WB,ICC/IF,IP,FCM。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 25 μL | ¥ 785 | 5日内发货 | |
| 50 μL | ¥ 1,390 | 5日内发货 | |
| 100 μL | ¥ 2,495 | 5日内发货 |
| 产品描述 | Anti-PCYT1A Antibody (4E85) is a Rabbit antibody targeting PCYT1A. Anti-PCYT1A Antibody (4E85) can be used in WB,ICC/IF,IP,FCM. |
| Ig Type | IgG |
| 克隆号 | 4E85 |
| 反应种属 | Human |
| 验证活性 | 1. 4% Paraformaldehyde-fixed HepG2 (H) cell; Triton X-100 at r. T. for 20 min; Antibody incubation with (PCYT1A) monoclonal Antibody, unconjugated (TMAB-10091) 1:100, 90 min at 37°C; followed by conjugated Goat Anti-Rabbit IgG antibody (green) at 37°C for 90 min, DAPI (blue) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control. 2. 25 μg total protein per lane of various lysates (see on figure) probed with PCYT1A monoclonal antibody, unconjugated (TMAB-10091) at 1: 2000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min. |
| 应用 | WBICC/IFIPFCM |
| 推荐剂量 | WB: 1:500-2000; ICC/IF: 1:50-200; IP: 1:20-50; FCM: 1:50-100 |
| 抗体种类 | Monoclonal |
| 宿主来源 | Rabbit |
| 亚细胞定位 | Cytoplasm; cytosol. Membrane. It can interconvert between an inactive cytosolic form and an active membrane-bound form. |
| 构建方式 | Recombinant Antibody |
| 纯化方式 | Protein A purified |
| 性状 | Liquid |
| 缓冲液 | 10mM phosphate buffered saline (pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol. |
| 研究背景 | Catalyzes the key rate-limiting step in the CDP-choline pathway for phosphatidylcholine biosynthesis. |
| 免疫原 | A synthesized peptide: human PCYT1A |
| 抗原种属 | Human |
| 基因名称 | PCYT1A |
| 基因ID | |
| 蛋白名称 | Choline-phosphate cytidylyltransferase A |
| Uniprot ID | |
| 功能 | Controls phosphatidylcholine synthesis. |
| 分子量 | Theoretical: 42 kDa. Actual: 42 kDa. |
| 储存方式 | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |