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Anti-PCNA Antibody (6Q348) 是一种抗体,靶向 PCNA。Anti-PCNA Antibody (6Q348) 可用于 ELISA, FC。
Anti-PCNA Antibody (6Q348) 是一种抗体,靶向 PCNA。Anti-PCNA Antibody (6Q348) 可用于 ELISA, FC。
规格 | 价格 | 库存 | 数量 |
---|---|---|---|
50 μL | ¥ 1,310 | 5日内发货 | |
100 μL | ¥ 2,185 | 5日内发货 |
产品描述 | Anti-PCNA Antibody (6Q348) is an antibody targeting PCNA. Anti-PCNA Antibody (6Q348) can be used in ELISA, FC. |
别名 | proliferating cell nuclear antigen, ATLD2 |
Ig Type | Mouse IgG2a |
克隆号 | 6Q348 |
交叉反应 | Human |
验证活性 | Overlay Peak curve showing JK cells stained with TMAH-00858 (red line) at 1:300. The cells were fixed in 4% formaldehyde (15min) and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum was Incubated to block non-specific protein-protein interactions followed by the antibody (1µg/1*10^6 cells) for 45 min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/200 dilution for 35 min at 4°C. Isotype control antibody (green line) was mouse IgG1 (1µg/1*10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed. |
应用 | ELISA, FC |
推荐剂量 | FC:1:20-1:500. |
抗体种类 | Monoclonal |
亚细胞定位 | Nucleus. |
构建方式 | Recombinant Antibody |
纯化方式 | Affinity-chromatography |
性状 | Liquid |
缓冲液 | Preservative: 0.03% Proclin 300. Constituents: 50% Glycerol, 0.01M PBS, PH 7.4. |
研究背景 | Auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2. Plays a key role in DNA damage response (DDR) by being conveniently positioned at the replication fork to coordinate DNA replication with DNA repair and DNA damage tolerance pathways. Acts as a loading platform to recruit DDR proteins that allow completion of DNA replication after DNA damage and promote postreplication repair: Monoubiquitinated PCNA leads to recruitment of translesion (TLS) polymerases, while 'Lys-63'-linked polyubiquitination of PCNA is involved in error-free pathway and employs recombination mechanisms to synthesize across the lesion. |
偶联 | Unconjugated |
免疫原 | Recombinant Protein: Human PCNA Protein |
抗原种属 | Human |
基因ID | 5111 |
Uniprot ID | |
研究领域 | Isotype/Loading Controls, Epigenetics and Nuclear Signaling, Cancer, Cell biology, Tags & Cell Markers |
储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
运输方式 | Shipping with blue ice. |
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