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Anti-PCK2 Antibody (4S45)
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产品编号 TMAC-03097
别名 phosphoenolpyruvate carboxykinase 2 (mitochondrial), PEPCK-M, PEPCK2, PEPCK
Anti-PCK2 Antibody (4S45) 是一种 Rabbit 抗体，靶向 PCK2。Anti-PCK2 Antibody (4S45) 可用于 WB,ICC,IF,IHC,FCM。
Anti-PCK2 Antibody (4S45)
Anti-PCK2 Antibody (4S45)
还可以产品编号 TMAC-03097 别名 phosphoenolpyruvate carboxykinase 2 (mitochondrial), PEPCK-M, PEPCK2, PEPCK
Anti-PCK2 Antibody (4S45) 是一种 Rabbit 抗体，靶向 PCK2。Anti-PCK2 Antibody (4S45) 可用于 WB,ICC,IF,IHC,FCM。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 50 μL | ¥ 1,480 | 5日内发货 | |
| 100 μL | ¥ 2,495 | 5日内发货 |
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产品介绍
生物活性
偶联与修饰
抗原信息
化学信息
| 产品描述 | Anti-PCK2 Antibody (4S45) is a Rabbit antibody targeting PCK2. Anti-PCK2 Antibody (4S45) can be used in WB,ICC,IF,IHC,FCM. |
| 别名 | phosphoenolpyruvate carboxykinase 2 (mitochondrial), PEPCK-M, PEPCK2, PEPCK |
| Ig Type | IgG |
| 交叉反应 | Human,Mouse,Rat |
| 验证活性 | 1. Western blot analysis of PCK2 on human kidney tissue (1) and MCF-7 cell (2) lysate using anti-PCK2 antibody at 1/500 dilution. 2. Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-PCK2 antibody. Counter stained with hematoxylin. 3. Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-PCK2 antibody. Counter stained with hematoxylin. 4. Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-PCK2 antibody. Counter stained with hematoxylin. 5. Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-PCK2 antibody. Counter stained with hematoxylin. 6. Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-PCK2 antibody. Counter stained with hematoxylin. 7. ICC staining PCK2 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 8. ICC staining PCK2 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 9. Flow cytometric analysis of MCF-7 cells with PCK2 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody. |
| 应用 | |
| 推荐剂量 | WB: 1:500-1000; IHC: 1:50-200; ICC: 1:50-100; FCM: 1:50-100 |
| 抗体种类 | Monoclonal |
| 宿主来源 | Rabbit |
| 构建方式 | Recombinant Antibody |
| 纯化方式 | ProA affinity purified |
| 性状 | Liquid |
| 缓冲液 | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| 研究背景 | Normal adjustment to changes in blood glucose levels depends on Insulin signaling as well as enzymes involved in the regulation of gluconeogenesis. Pathological changes to this process are central to the type 2 diabetes phenotype. Phosphoenolpyruvate carboxykinase (PEPCK) plays an important role in this process by stimulating hepatic glucose production. PEPCK expression increases in response to glucagon and glucocorticoids, while Insulin suppresses expression. Modulation of the signals governing PEPCK levels present a potential therapeutic approach to the treatment of Insulin resistance and consequently obesity. The cytosolic form of PEPCK, known as PEPCK-C, and the mitochondrial form, known as PEPCK-M, are encoded by two different nuclear genes in mouse, human and chicken. |
| 偶联 | Unconjugated |
| 免疫原 | Recombinant Protein |
| Uniprot ID |
| 分子量 | Theoretical: 71 kDa. |
存储&运输
| 储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |
计算器
比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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