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Anti-PCDH7 Polyclonal Antibody 2 是一种 Rabbit 抗体,靶向 PCDH7。Anti-PCDH7 Polyclonal Antibody 2 可用于 WB,IHC-P,IHC-Fr,IF。
Anti-PCDH7 Polyclonal Antibody 2 是一种 Rabbit 抗体,靶向 PCDH7。Anti-PCDH7 Polyclonal Antibody 2 可用于 WB,IHC-P,IHC-Fr,IF。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 50 μL | ¥ 1,160 | 5日内发货 | |
| 100 μL | ¥ 1,965 | 5日内发货 | |
| 200 μL | ¥ 2,795 | 5日内发货 |
| 产品描述 | Anti-PCDH7 Polyclonal Antibody 2 is a Rabbit antibody targeting PCDH7. Anti-PCDH7 Polyclonal Antibody 2 can be used in WB,IHC-P,IHC-Fr,IF. |
| Ig Type | IgG |
| 反应种属 | Human,Mouse (predicted:Rat,Dog,Pig,Cow,Horse,Sheep) |
| 验证活性 | 1. Paraformaldehyde-fixed, paraffin embedded (Human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (PCDH7) Polyclonal Antibody, Unconjugated (TMAB-10050) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining. 2. Sample: Hela (Human) Cell Lysate at 30 μg HepG2 (Human) Cell Lysate at 30 μg MCF-7 (Human) Cell Lysate at 30 μg NIH/3T3 (Mouse) Cell Lysate at 30 μg Primary: Anti-PCDH7 (TMAB-10050) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 113 kD Observed band size: 150 kD |
| 应用 | WBIHC-PIHC-FrIF |
| 推荐剂量 | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| 抗体种类 | Polyclonal |
| 宿主来源 | Rabbit |
| 亚细胞定位 | Cell membrane. |
| 构建方式 | Polyclonal Antibody |
| 纯化方式 | Protein A purified |
| 性状 | Liquid |
| 缓冲液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 浓度 | 1mg/ml |
| 研究背景 | As a subfamily of the cadherin superfamily, protocadherins are cadherin-like cell adhesion proteins that contain up to seven extracellular domains and are predominantly expressed in the nervous system. Importantly, the adhesion mechanism of protocadherins is distinct from classic cadherins. Through inactivation or overexpression, several protocadherins have been implicated in a variety of cancers. PCDH7 (protocadherin 7), also known as BHPCDH or BH-Pcdh, is a 1069 amino acid single-pass I membrane protein that is expressed in the brain and heart. Containing seven cadherin domains, PCDH7 is thought to function in cell-cell recognition and adhesion. PCDH7 exists as three isoforms due to alternative splicing events. |
| 免疫原 | KLH conjugated synthetic peptide: human PCDH7 |
| 抗原种属 | Human |
| 基因名称 | PCDH7 |
| 基因ID | |
| 蛋白名称 | Protocadherin-7 |
| Uniprot ID | |
| 功能 | Expressed predominantly in brain and heart and at lower levels in various other tissues. |
| 分子量 | Theoretical: 113 kDa. |
| 储存方式 | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |