Anti-PBK Polyclonal Antibody is a Rabbit antibody targeting PBK. Anti-PBK Polyclonal Antibody can be used in WB,FCM.

IgG

Human,Mouse (predicted:Rat,Dog,Pig,Cow)

1. Sample:
Testis (Mouse) Lysate at 40 μg
Primary: Anti-PBK (TMAB-10025) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 36 kD
Observed band size: 37 kD
2. Blank control (black line): HepG2.
Primary Antibody (green line): Rabbit Anti-PBK antibody (TMAB-10025)
Dilution: 2 μg/Test;
Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC
Dilution: 0.5 μg/Test.
Isotype control (orange line): Normal Rabbit IgG
Protocol
The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

WB: 1:500-2000; FCM: 2μg/Test

Polyclonal

KLH conjugated synthetic peptide: human PBK

Human

PBK

Lymphokine-activated killer T-cell-originated protein kinase

Phosphorylates MAP kinase p38. Seems to be active only in mitosis. May also play a role in the activation of lymphoid cells. When phosphorylated, forms a complex with TP53, leading to TP53 destabilization and attenuation of G2/M checkpoint during doxorubicin-induced DNA damage.