Anti-PAX8 Polyclonal Antibody is a Rabbit antibody targeting PAX8. Anti-PAX8 Polyclonal Antibody can be used in IHC-P,IHC-Fr,ICC/IF,IF,FCM.

IgG

Human,Rat (predicted:Mouse,Dog,Cow,Sheep,Goat)

1. U87MG cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Antibody incubation with (PAX8) polyclonal Antibody, Unconjugated (TMAB-10018) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.
2. Primary Antibody: Rabbit Anti-TLR4 antibody;
Isotype Control Antibody: Rabbit IgG (orange line);
Secondary Antibody: F (ab’)2 fragment goat anti-rabbit IgG-PE (white blue line)
Positive control: Jurkat cells (blue line)
Protocol
1.Jurkat cells were washed twice with phosphate-buffered saline (PBS).
2. An equivalent amount of pre-warmed 4% paraformaldehyde was added and the cells were incubated for 1 0 min at 37 °C.
3. Tissue/cell: rat colon tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37℃ for 20 min;
Incubation: Anti-PAX8 Polyclonal Antibody, Unconjugated (TMAB-10018) 1: 200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining
4. Cell: HT-29;
Concentration: 1:100
Host/Isotype: Rabbit/Igg (blue line)
Flow cytometric analysis of Rabbit IgG isotype control on HT-29 (blue) compared with primary antibodY followed by Alexa Fluor 488-conjugated goat anti-rabbit Igg (H+L) secondary antibody.

IHC-P: 1:100-500; IHC-Fr: 1:100-500; ICC/IF: 1:100-500; IF: 1:100-500; FCM: 1μg/Test

Polyclonal

KLH conjugated synthetic peptide: human PAX8

Human

PAX8

Paired box protein Pax-8

Transcription factor for the thyroid-specific expression of the genes exclusively expressed in the thyroid cell type, maintaining the functional differentiation of such cells.