Anti-PAK2 Antibody (5W642) is an antibody targeting PAK2. Anti-PAK2 Antibody (5W642) can be used in ELISA, WB, FC, IP.

Rabbit IgG

5W642

Human, Mouse, Rat

1. Western Blot
-Positive WB detected in: 293 whole cell lysate, Jurkat whole cell lysate, Raji whole cell lysate, Mouse brain tissue, Rat brain tissue
-All lanes: PAK2 antibody at 1:2000
-Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution
-Predicted band size: 59 kDa
-Observed band size: 59 kDa
2. Overlay histogram showing HepG2 cells stained with TMAH-00851 (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then incubated in 10% normal goat serum to block non-specific protein-protein interactions followedby the antibody (1µg/1*10^6 cells) for 1 h at 4°C.The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 30min at 4°C. Control antibody (green line) was Rabbit IgG (1µg/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.
3. Immunoprecipitating PAK2 in Raji whole cell lysate
-Lane 1: Rabbit control IgG instead of TMAH-00851 in Raji whole cell lysate.
For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
-Lane 2: TMAH-00851(2µg)+ Raji whole cell lysate(500µg)
-Lane 3: Raji whole cell lysate (10µg)

ELISA, WB, FC, IP

WB:1:500-1:5000; FC:1:20-1:200; IP:1:200-1:1000.

Monoclonal

Unconjugated

A synthetic peptide: Human PAK2

Human

5062

Neuroscience, Cancer, Cell biology, Microbiology, Signal transduction