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Anti-PAI1 Antibody (8G921) 是一种 Rabbit 抗体,靶向 PAI1。Anti-PAI1 Antibody (8G921) 可用于 FCM, WB。
Anti-PAI1 Antibody (8G921) 是一种 Rabbit 抗体,靶向 PAI1。Anti-PAI1 Antibody (8G921) 可用于 FCM, WB。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 25 μL | ¥ 790 | 5日内发货 | |
| 50 μL | ¥ 1,395 | 5日内发货 | |
| 100 μL | ¥ 2,495 | 5日内发货 |
| 产品描述 | Anti-PAI1 Antibody (8G921) is a Rabbit antibody targeting PAI1. Anti-PAI1 Antibody (8G921) can be used in FCM, WB. |
| Ig Type | IgG |
| 克隆号 | 8G921 |
| 反应种属 | Human |
| 验证活性 | 1. 25 ug total protein per lane of various lysates (see on figure) probed with PAI1 monoclonal antibody, unconjugated (TMAB-09935) at 1: 1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min. 2. The HUVEC (H) cells were fixed with 4% PFA (10 min at r. T.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, the cells then were incubated in 5% BSA to block non-specific protein-protein interactions (30 min at r. T.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green): Rabbit Anti-PAI1 antibody (TMAB-09935, 1: 100); Isotype Control (orange): Rabbit IgG. Blank control (black): PBS. Acquisition of 20,000 events was performed. |
| 应用 | FCMWB |
| 推荐剂量 | FCM=1:50-100; WB=1:500-2000 |
| 抗体种类 | Monoclonal |
| 宿主来源 | Rabbit |
| 亚细胞定位 | Secreted |
| 组织特异性 | Found in plasma and platelets and in endothelial, hepatoma and fibrosarcoma cells |
| 构建方式 | Recombinant Antibody |
| 纯化方式 | Protein A purified |
| 性状 | Liquid |
| 缓冲液 | 50mM Tris-Glycine (pH7.4), 0.15M NaCl, 40% Glycerol, 0.02% Proclin300 and 0.05% BSA. |
| 浓度 | 1mg/ml |
| 研究背景 | PAI1 (plasminogen activator inhibitor 1) is originally cloned from human endothelial cell (Pannekoek 1986, Ginsburg 1986) and rat hepatoma cell 3 cDNA libraries. As a member of the serpin family of serine protease inhibitors, PAI1 inhibits both tissue type plasminogen activator (tPA) and urokinase type plasminogen activator (uPA). High PAI1 levels are associated with an increased risk of thromboembolic disease while PAI1 deficiency may represent an inherited autosomal recessive bleeding disorder. |
| 免疫原 | A synthesized peptide: human PAI 1 |
| 抗原种属 | Human |
| 基因名称 | SERPINE1 |
| 基因ID | |
| 蛋白名称 | Plasminogen activator inhibitor 1 |
| Uniprot ID | |
| 功能 | Serine protease inhibitor. This inhibitor acts as 'bait' for tissue plasminogen activator, urokinase, protein C and matriptase-3/TMPRSS7. Its rapid interaction with PLAT may function as a major control point in the regulation of fibrinolysis. |
| 分子量 | Theoretical: 43 kDa. |
| 储存方式 | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |