Anti-PAI-1 Polyclonal Antibody is a Rabbit antibody targeting PAI-1. Anti-PAI-1 Polyclonal Antibody can be used in WB,ICC/IF.

IgG

Human,Mouse,Rat (predicted:Dog,Pig,Cow,Rabbit,Sheep)

1. A549 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Antibody incubation with (PAI1) polyclonal Antibody, Unconjugated (TMAB-01327) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nucleus.
2. A549 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Antibody incubation with (PAI1) polyclonal Antibody, Unconjugated (TMAB-01327) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nucleus.
3. Sample:
Lane 1: Mouse Lung tissue lysates
Lane 2: Rat Lung tissue lysates
Primary: Anti-PAI1 (TMAB-01327) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 43 kDa
Observed band size: 46 kDa

WB: 1:500-2000; ICC/IF: 1:100

Polyclonal

KLH conjugated synthetic peptide: human PAI-1

Human