Anti-p53BP1 Polyclonal Antibody is a Rabbit antibody targeting p53BP1. Anti-p53BP1 Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF,ICC/IF.

IgG

Human,Rat (predicted:Mouse,Dog,Rabbit)

1. Tissue/cell: rat colon tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-p53BP1 Polyclonal Antibody, Unconjugated (TMAB-01322) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
2. 4% Paraformaldehyde-fixed Hela (H) cell; Triton X-100 at RT for 20 min; Antibody incubation with (p53BP1) polyclonal Antibody, unconjugated (TMAB-01322) 1:100, 90 min at 37°C; followed by conjugated Goat Anti-Rabbit IgG antibody (green ) at 37°C for 90 min, DAPI (blue) was used to stain the cell nucleus. PBS instead of the primary antibody was used as the blank control.

IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; ICC/IF: 1:50-1:200

Polyclonal

KLH conjugated synthetic peptide: human p53BP1/53BP1

Human