Anti-P2Y8 Polyclonal Antibody is a Rabbit antibody targeting P2Y8. Anti-P2Y8 Polyclonal Antibody can be used in WB,FCM.

IgG

Human

1. Blank control: HL60.
Primary Antibody (green line): Rabbit Anti-P2Y8 antibody (TMAB-09864)
Dilution: 2 μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody (blue line): Goat anti-rabbit IgG-AF488
Dilution: 1 μg /test.
Protocol
The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
2. Blank control: Raji.
Primary Antibody (green line): Rabbit Anti-P2Y8 antibody (TMAB-09864)
Dilution: 2 μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody: Goat anti-rabbit IgG-AF488
Dilution: 1 μg /test.
Protocol
The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
3. Sample: Raji Cell (Human) Lysate at 30 μg
Primary: Anti-P2Y8 (TMAB-09864) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 66 kD
Observed band size: 50 kD

WB: 1:500-2000; FCM: 2μg/Test

Polyclonal

KLH conjugated synthetic peptide: human P2Y8

Human

P2RY8

P2Y purinoceptor 8

Probable receptor for purines coupled to G-proteins.