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Anti-p21 Polyclonal Antibody

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货号 TMAB-01312

别名 Wildtype p53 Activating Fragment 1, WAF1, SLC12A9, SDI1, PIC1, p21WAF, p21CIP1, P21 protein, Melanoma differentiation-associated protein, Melanoma Differentiation Associated Protein 6, MDA6, MDA 6, DNA Synthesis Inhibitor, Cyclin-dependent kinase inhibitor 1A (p21, Cip1), Cyclin-dependent kinase inhibitor 1A (P21), Cyclin-dependent kinase inhibitor 1, Cyclin Dependent Kinase Inhibitor 1A, CIP1, CDN1A, CDKN1A, CDKN1, CDKN 1, CDK-interacting protein 1, CDKI, Cation chloride cotransporter-interacting protein 1, CAP20, Activating Fragment 1

Anti-p21 Polyclonal Antibody 是一种 Rabbit 抗体，靶向 p21。Anti-p21 Polyclonal Antibody 可用于 IF,IHC-Fr,IHC-P,WB。

Anti-p21 Polyclonal Antibody

Anti-p21 Polyclonal Antibody

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货号 TMAB-01312 别名 Wildtype p53 Activating Fragment 1, WAF1, SLC12A9, SDI1, PIC1, p21WAF, p21CIP1, P21 protein, Melanoma differentiation-associated protein, Melanoma Differentiation Associated Protein 6, MDA6, MDA 6, DNA Synthesis Inhibitor, Cyclin-dependent kinase inhibitor 1A (p21, Cip1), Cyclin-dependent kinase inhibitor 1A (P21), Cyclin-dependent kinase inhibitor 1, Cyclin Dependent Kinase Inhibitor 1A, CIP1, CDN1A, CDKN1A, CDKN1, CDKN 1, CDK-interacting protein 1, CDKI, Cation chloride cotransporter-interacting protein 1, CAP20, Activating Fragment 1

Anti-p21 Polyclonal Antibody 是一种 Rabbit 抗体，靶向 p21。Anti-p21 Polyclonal Antibody 可用于 IF,IHC-Fr,IHC-P,WB。

规格价格库存数量
50 μL
¥ 1,170
5日内发货
100 μL
¥ 1,965
5日内发货
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产品介绍

生物活性
产品描述
Anti-p21 Polyclonal Antibody is a Rabbit antibody targeting p21. Anti-p21 Polyclonal Antibody can be used in IF,IHC-Fr,IHC-P,WB.
别名Wildtype p53 Activating Fragment 1, WAF1, SLC12A9, SDI1, PIC1, p21WAF, p21CIP1, P21 protein, Melanoma differentiation-associated protein, Melanoma Differentiation Associated Protein 6, MDA6, MDA 6, DNA Synthesis Inhibitor, Cyclin-dependent kinase inhibitor 1A (p21, Cip1), Cyclin-dependent kinase inhibitor 1A (P21), Cyclin-dependent kinase inhibitor 1, Cyclin Dependent Kinase Inhibitor 1A, CIP1, CDN1A, CDKN1A, CDKN1, CDKN 1, CDK-interacting protein 1, CDKI, Cation chloride cotransporter-interacting protein 1, CAP20, Activating Fragment 1
Ig Type
IgG
反应种属
Human (predicted:Mouse,Rat)
验证活性
1. Paraformaldehyde-fixed, paraffin embedded (human breast cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (p21) Polyclonal Antibody, Unconjugated (TMAB-01312) at 1:100 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
2. Sample:
Lane 1: MCF-7 (Human) Cell Lysate at 30 μg
Lane 2: Hela (Human) Cell Lysate at 30 μg
Lane 3: Huvec (Human) Cell Lysate at 30 μg
Lane 4: U87MG (Human) Cell Lysate at 30 μg
Lane 5: 293T (Human) Cell Lysate at 30 μg
Lane 6: A673 (Human) Cell Lysate at 30 μg
Primary:
Anti-p21 (TMAB-01312) at 1/1000 dilution
Anti-beta-Actin at 1/2000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 21 kDa
Observed band size: 21 kDa
verifiedActivityverifiedActivity
应用IFIHC-FrIHC-PWB
推荐剂量
WB: 1:500-2000; IHC-P: 1:20-100; IHC-Fr: 1:20-100; IF: 1:100-500
抗体种类
Polyclonal
宿主来源Rabbit
亚细胞定位Cytoplasmic and Nuclear.
组织特异性Expressed in all adult tissues, with 5-fold lower levels observed in the brain.
构建方式Polyclonal Antibody
纯化方式Protein A purified
性状Liquid
缓冲液0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
浓度1 mg/mL
研究背景This gene encodes a potent cyclin-dependent kinase inhibitor. The encoded protein binds to and inhibits the activity of cyclin-CDK2 or -CDK4 complexes, and thus functions as a regulator of cell cycle progression at G1. The expression of this gene is tightly controlled by the tumor suppressor protein p53, through which this protein mediates the p53-dependent cell cycle G1 phase arrest in response to a variety of stress stimuli. This protein can interact with proliferating cell nuclear antigen (PCNA), a DNA polymerase accessory factor, and plays a regulatory role in S phase DNA replication and DNA damage repair. This protein was reported to be specifically cleaved by CASP3-like caspases, which thus leads to a dramatic activation of CDK2, and may be instrumental in the execution of apoptosis following caspase activation. Two alternatively spliced variants, which encode an identical protein, have been reported. Two families of cyclin dependent kinase inhibitors (CKIs) have been identified. The p21WAF1/Cip1 family inhibits all kinases involved in the G1/S transition. The p16INK4a family inhibits Cdk4 and Cdk6 specifically.
抗原信息
免疫原
Recombinant Protein: human p21
抗原种属
Human
基因名称
CDKN1A
基因ID
1026
蛋白名称
Cyclin-dependent kinase inhibitor 1
Uniprot ID
P38936
研究领域
Cip / Kip,Cip/Kip,DNA / RNA binding,Co-activators/co-repressors,Zinc Finger,Co-activators/co-repressors
功能
May be the important intermediate by which p53/TP53 mediates its role as an inhibitor of cellular proliferation in response to DNA damage. Binds to and inhibits cyclin-dependent kinase activity, preventing phosphorylation of critical cyclin-dependent kinase substrates and blocking cell cycle progression. Functions in the nuclear localization and assembly of cyclin D-CDK4 complex and promotes its kinase activity towards RB1. At higher stoichiometric ratios, inhibits the kinase activity of the cyclin D-CDK4 complex.
化学信息
分子量Theoretical: 18 kDa.
储存&溶解度
储存方式Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
运输方式Shipping with blue ice.
存储store at low temperature | store at -20°C

计算器

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