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Anti-p21 Polyclonal Antibody
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产品编号 TMAB-01312
Anti-p21 Polyclonal Antibody 是一种 Rabbit 抗体，靶向 p21。Anti-p21 Polyclonal Antibody 可用于 WB,IHC-P,IHC-Fr,IF。
Anti-p21 Polyclonal Antibody
Anti-p21 Polyclonal Antibody
还可以产品编号 TMAB-01312
Anti-p21 Polyclonal Antibody 是一种 Rabbit 抗体，靶向 p21。Anti-p21 Polyclonal Antibody 可用于 WB,IHC-P,IHC-Fr,IF。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 50 μL | ¥ 1,170 | 5日内发货 | |
| 100 μL | ¥ 1,965 | 5日内发货 |
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产品介绍
生物活性
抗原信息
| 产品描述 | Anti-p21 Polyclonal Antibody is a Rabbit antibody targeting p21. Anti-p21 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF. |
| Ig Type | IgG |
| 交叉反应 | Human (predicted:Mouse,Rat) |
| 验证活性 | 1. Paraformaldehyde-fixed, paraffin embedded (human breast cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (p21) Polyclonal Antibody, Unconjugated (TMAB-01312) at 1:100 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 2. Sample: Lane 1: MCF-7 (Human) Cell Lysate at 30 μg Lane 2: Hela (Human) Cell Lysate at 30 μg Lane 3: Huvec (Human) Cell Lysate at 30 μg Lane 4: U87MG (Human) Cell Lysate at 30 μg Lane 5: 293T (Human) Cell Lysate at 30 μg Lane 6: A673 (Human) Cell Lysate at 30 μg Primary: Anti-p21 (TMAB-01312) at 1/1000 dilution Anti-beta-Actin at 1/2000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 21 kDa Observed band size: 21 kDa |
| 应用 | |
| 推荐剂量 | WB: 1:500-2000; IHC-P: 1:20-100; IHC-Fr: 1:20-100; IF: 1:100-500 |
| 抗体种类 | Polyclonal |
| 宿主来源 | Rabbit |
| 亚细胞定位 | Cytoplasmic and Nuclear. |
| 构建方式 | Hybridoma Polyclonal Antibody |
| 纯化方式 | Protein A purified |
| 性状 | Liquid |
| 缓冲液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究背景 | This gene encodes a potent cyclin-dependent kinase inhibitor. The encoded protein binds to and inhibits the activity of cyclin-CDK2 or -CDK4 complexes, and thus functions as a regulator of cell cycle progression at G1. The expression of this gene is tightly controlled by the tumor suppressor protein p53, through which this protein mediates the p53-dependent cell cycle G1 phase arrest in response to a variety of stress stimuli. This protein can interact with proliferating cell nuclear antigen (PCNA), a DNA polymerase accessory factor, and plays a regulatory role in S phase DNA replication and DNA damage repair. This protein was reported to be specifically cleaved by CASP3-like caspases, which thus leads to a dramatic activation of CDK2, and may be instrumental in the execution of apoptosis following caspase activation. Two alternatively spliced variants, which encode an identical protein, have been reported. Two families of cyclin dependent kinase inhibitors (CKIs) have been identified. The p21WAF1/Cip1 family inhibits all kinases involved in the G1/S transition. The p16INK4a family inhibits Cdk4 and Cdk6 specifically. |
| 免疫原 | Recombinant Protein: human p21 |
| 抗原种属 | Human |
| 基因ID | |
| Uniprot ID | |
| 研究领域 | Cip / Kip,Cip/Kip,DNA / RNA binding,Co-activators/co-repressors,Zinc Finger,Co-activators/co-repressors |
存储&运输
| 储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |
计算器
比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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