Anti-Osteoprotegerin Polyclonal Antibody 2 is a Rabbit antibody targeting Osteoprotegerin. Anti-Osteoprotegerin Polyclonal Antibody 2 can be used in WB,IHC-P,IHC-Fr,IF.

IgG

Human,Mouse,Rat (predicted:Dog,Rabbit)

1. Paraformaldehyde-fixed, paraffin embedded (Rat stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (OPG) Polyclonal Antibody, Unconjugated (TMAB-01301) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
2. Paraformaldehyde-fixed, paraffin embedded (Rat heart); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (OPG) Polyclonal Antibody, Unconjugated (TMAB-01301) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
3. Sample:
Lane 1: Raji (Human) Cell Lysate at 30 μg
Lane 2: U2os (Human) Cell Lysate at 30 μg
Lane 3: Heart (Mouse) Lysate at 40 μg
Lane 4: Heart (Rat) Lysate at 40 μg
Lane 5: Liver (Mouse) Lysate at 40 μg
Lane 6: Liver (Rat) Lysate at 40 μg
Lane 7: Kidney (Mouse) Lysate at 40 μg
Lane 8: Kidney (Rat) Lysate at 40 μg
Primary: Anti-OPG (TMAB-01301) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 55 kDa
Observed band size: 55 kDa

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human OPG

Human

Inflammatory mediators,Blood,Bone,TNF Superfamily,Obesity,Bone,TNF