Anti-Osteocalcin Polyclonal Antibody 2 is a Rabbit antibody targeting Osteocalcin. Anti-Osteocalcin Polyclonal Antibody 2 can be used in ELISA,IF,IHC-Fr,IHC-P,WB.

IgG

Human,Mouse (predicted:Rat)

1. Sample:
Lane 1: Mouse NIH/3T3 cell lysates
Lane 2: Human MG63 cell lysates
Lane 3: Human A549 cell lysates
Lane 4: Human U-2 OS cell lysates
Primary: Anti-Osteocalcin (TMAB-01296) at 1/500 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 11 kDa
Observed band size: 11 kDa
2. Paraformaldehyde-fixed, paraffin embedded (human skeletal muscle); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (Osteocalcin) Polyclonal Antibody, Unconjugated (TMAB-01296) at 1:100 overnight at 4°C, followed by a conjugated Goat Anti-Rabbit IgG antibody for 90 minutes, and DAPI for nucleus staining.
3. Paraformaldehyde-fixed, paraffin embedded (human skeletal muscle); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (Osteocalcin) Polyclonal Antibody, Unconjugated (TMAB-01296) at 1:100 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; ELISA: 1:5000-10000

Polyclonal

KLH conjugated synthetic peptide: human Osteocalcin

Human

BGLAP

Osteocalcin

Bone,Osteogenesis

Constitutes 1-2% of the total bone protein. It binds strongly to apatite and calcium.