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Anti-Osteoactivin/GPNMB Polyclonal Antibody

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货号 TMAB-01294

别名 Osteoactivin, NMB, HGFIN, glycoprotein (transmembrane) nmb

Anti-Osteoactivin/GPNMB Polyclonal Antibody 是一种 Rabbit 抗体，靶向 Osteoactivin/GPNMB。Anti-Osteoactivin/GPNMB Polyclonal Antibody 可用于 IF,IHC-Fr,IHC-P,WB。

Anti-Osteoactivin/GPNMB Polyclonal Antibody

Anti-Osteoactivin/GPNMB Polyclonal Antibody

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货号 TMAB-01294 别名 Osteoactivin, NMB, HGFIN, glycoprotein (transmembrane) nmb

Anti-Osteoactivin/GPNMB Polyclonal Antibody 是一种 Rabbit 抗体，靶向 Osteoactivin/GPNMB。Anti-Osteoactivin/GPNMB Polyclonal Antibody 可用于 IF,IHC-Fr,IHC-P,WB。

规格价格库存数量
50 μL
¥ 1,165
5日内发货
100 μL
¥ 1,960
5日内发货
200 μL
¥ 2,795
5日内发货
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产品介绍

生物活性
产品描述
Anti-Osteoactivin/GPNMB Polyclonal Antibody is a Rabbit antibody targeting Osteoactivin/GPNMB. Anti-Osteoactivin/GPNMB Polyclonal Antibody can be used in IF,IHC-Fr,IHC-P,WB.
别名Osteoactivin, NMB, HGFIN, glycoprotein (transmembrane) nmb
Ig Type
IgG
反应种属
Human,Rat (predicted:Pig,Cow,Horse,GuineaPig)
验证活性
1. Tissue/cell: rat lung tissue (melanoma); 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-GPNMB/Osteoactivin Polyclonal Antibody, Unconjugated (TMAB-01294) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
2. Sample:
Lane 1: Recombinant human GPNMB protein, C-His (HEK293)
Primary: Anti-GPNMB (TMAB-01294) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 61 kDa
Observed band size: 65 kDa
verifiedActivityverifiedActivity
应用IFIHC-FrIHC-PWB
推荐剂量
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500
抗体种类
Polyclonal
宿主来源Rabbit
亚细胞定位Membrane; Single-pass type I membrane protein (Potential). Melanosome. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
组织特异性Not restricted to the melanocytic lineage.
构建方式Polyclonal Antibody
纯化方式Protein A purified
性状Liquid
缓冲液0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
浓度1 mg/mL
研究背景The protein encoded by this gene is a type I transmembrane glycoprotein which shows homology to the pMEL17 precursor, a melanocyte-specific protein. GPNMB shows expression in the lowly metastatic human melanoma cell lines and xenografts but does not show expression in the highly metastatic cell lines. GPNMB may be involved in growth delay and reduction of metastatic potential. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
抗原信息
免疫原
KLH conjugated synthetic peptide: human GPNMB
抗原种属
Human
基因名称
GPNMB
基因ID
10457
蛋白名称
Transmembrane glycoprotein NMB
Uniprot ID
Q14956
研究领域
Tumor-associated antigens,Macrophage / Inflamm.
功能
Could be a melanogenic enzyme.
化学信息
分子量Theoretical: 61 kDa.
颜色Transparent
物理性状Liquid
储存&溶解度
储存方式Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
运输方式Shipping with blue ice.
存储store at low temperature | store at -20°C

计算器

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