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Anti-Osteoactivin/GPNMB Polyclonal Antibody 是一种 Rabbit 抗体,靶向 Osteoactivin/GPNMB。Anti-Osteoactivin/GPNMB Polyclonal Antibody 可用于 WB,IHC-P,IHC-Fr,IF。
Anti-Osteoactivin/GPNMB Polyclonal Antibody 是一种 Rabbit 抗体,靶向 Osteoactivin/GPNMB。Anti-Osteoactivin/GPNMB Polyclonal Antibody 可用于 WB,IHC-P,IHC-Fr,IF。
规格 | 价格 | 库存 | 数量 |
---|---|---|---|
50 μL | ¥ 1,165 | 5日内发货 | |
100 μL | ¥ 1,960 | 5日内发货 | |
200 μL | ¥ 2,795 | 5日内发货 |
产品描述 | Anti-Osteoactivin/GPNMB Polyclonal Antibody is a Rabbit antibody targeting Osteoactivin/GPNMB. Anti-Osteoactivin/GPNMB Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF. |
Ig Type | IgG |
交叉反应 | Human,Rat (predicted:Pig,Cow,Horse,GuineaPig) |
验证活性 | 1. Tissue/cell: rat lung tissue (melanoma); 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Incubation: Anti-GPNMB/Osteoactivin Polyclonal Antibody, Unconjugated (TMAB-01294) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining. 2. Sample: Lane 1: Recombinant human GPNMB protein, C-His (HEK293) Primary: Anti-GPNMB (TMAB-01294) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 61 kDa Observed band size: 65 kDa |
应用 | |
推荐剂量 | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
抗体种类 | Polyclonal |
宿主来源 | Rabbit |
亚细胞定位 | Membrane; Single-pass type I membrane protein (Potential). Melanosome. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV. |
构建方式 | Hybridoma Polyclonal Antibody |
纯化方式 | Protein A purified |
性状 | Liquid |
缓冲液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
研究背景 | The protein encoded by this gene is a type I transmembrane glycoprotein which shows homology to the pMEL17 precursor, a melanocyte-specific protein. GPNMB shows expression in the lowly metastatic human melanoma cell lines and xenografts but does not show expression in the highly metastatic cell lines. GPNMB may be involved in growth delay and reduction of metastatic potential. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
免疫原 | KLH conjugated synthetic peptide: human GPNMB |
抗原种属 | Human |
基因ID | |
Uniprot ID | |
研究领域 | Tumor-associated antigens,Macrophage / Inflamm. |
储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
运输方式 | Shipping with blue ice. |
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