Anti-OPA1 Polyclonal Antibody is a Rabbit antibody targeting OPA1. Anti-OPA1 Polyclonal Antibody can be used in IF,IHC-Fr,IHC-P,WB.

IgG

Rat (predicted:Human,Mouse,Dog,Pig,Cow,Horse,Rabbit,Sheep)

1. Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-OPA1 Polyclonal Antibody, Unconjugated (TMAB-01288) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
2. Sample:
Cerebrum (Rat) Lysate at 40 μg
Primary: Anti-OPA1 (TMAB-01288) at 1/2000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 111 kDa
Observed band size: 111 kDa

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human OPA1

Human

OPA1

Dynamin-like 120 kDa protein, mitochondrial

Cardiac metabolism,Mitophagy fission and fusion,Neurodegenerative disease,Visual system

Dynamin-related GTPase required for mitochondrial fusion and regulation of apoptosis. May form a diffusion barrier for proteins stored in mitochondrial cristae. Proteolytic processing in response to intrinsic apoptotic signals may lead to disassembly of OPA1 oligomers and release of the caspase activator cytochrome C (CYCS) into the mitochondrial intermembrane space.