Anti-NXF1 Antibody (8Z751) is a Rabbit antibody targeting NXF1. Anti-NXF1 Antibody (8Z751) can be used in FCM, ICC/IF, IF, IHC-Fr, IHC-P, IP, WB.

IgG

8Z751

Human

1. 25 ug total protein per lane of various lysates (see on figure) probed with NXF1 monoclonal antibody, unconjugated (TMAB-09720) at 1: 1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.
2. The Hela (H) cells were fixed with 4% PFA (10 min at r. T.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, the cells then were incubated in 5% BSA to block non-specific protein-protein interactions (30 min at r. T.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green): Rabbit Anti-NXF1 antibody (TMAB-09720, 1: 100); Isotype Control (orange): Rabbit IgG. Blank control (black): PBS. Acquisition of 20,000 events was performed.

FCM=1:50-100; ICC/IF=1:50-200; IF=1:50-200; IHC-Fr=1:100-200; IHC-P=1:100-200; IP=1:20-50; WB=1:500-2000

Monoclonal

A synthesized peptide: human NXF1

Human

NXF1

Nuclear RNA export factor 1

Involved in the nuclear export of mRNA species bearing retroviral constitutive transport elements (CTE) and in the export of mRNA from the nucleus to the cytoplasm. The NXF1-NXT1 heterodimer is involved in the export of HSP70 mRNA in conjunction with THOC4 and THOC5.