Anti-NUP107 Antibody (3N675) is a Rabbit antibody targeting NUP107. Anti-NUP107 Antibody (3N675) can be used in FCM, WB.

IgG

3N675

Human

1. 25 ug total protein per lane of various lysates (see on figure) probed with NUP107 monoclonal antibody, unconjugated (TMAB-09702) at 1: 1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.
2. The Hela (H) cells were fixed with 4% PFA (10 min at r. T.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, the cells then were incubated in 5% BSA to block non-specific protein-protein interactions (30 min at r. T.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green): Rabbit Anti-NUP107 antibody (TMAB-09702, 1: 100); Isotype Control (orange): Rabbit IgG. Blank control (black): PBS. Acquisition of 20,000 events was performed.

FCM=1:50-100; WB=1:500-2000

Monoclonal

A synthesized peptide: human NUP107

Human

NUP107

Nuclear pore complex protein Nup107

Essential component of nuclear pore complex. Required for the assembly of peripheral proteins into the nuclear pore complex.