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Anti-NUMB Antibody (1M861)
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产品编号 TMAC-02905
别名 S171, Protein S171, Protein numb homolog, NUMB_HUMAN, Numb protein homolog, Numb homolog (Drosophila), Numb homolog, Numb, h-Numb, h Numb, FLJ31314, C14orf41, c14_5527
Anti-NUMB Antibody (1M861) 是一种 Rabbit 抗体，靶向 NUMB。Anti-NUMB Antibody (1M861) 可用于 WB,ICC,IHC。
Anti-NUMB Antibody (1M861)
Anti-NUMB Antibody (1M861)
还可以产品编号 TMAC-02905 别名 S171, Protein S171, Protein numb homolog, NUMB_HUMAN, Numb protein homolog, Numb homolog (Drosophila), Numb homolog, Numb, h-Numb, h Numb, FLJ31314, C14orf41, c14_5527
Anti-NUMB Antibody (1M861) 是一种 Rabbit 抗体，靶向 NUMB。Anti-NUMB Antibody (1M861) 可用于 WB,ICC,IHC。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 50 μL | ¥ 1,485 | 5日内发货 | |
| 100 μL | ¥ 2,480 | 5日内发货 |
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| 产品描述 | Anti-NUMB Antibody (1M861) is a Rabbit antibody targeting NUMB. Anti-NUMB Antibody (1M861) can be used in WB,ICC,IHC. |
| 别名 | S171, Protein S171, Protein numb homolog, NUMB_HUMAN, Numb protein homolog, Numb homolog (Drosophila), Numb homolog, Numb, h-Numb, h Numb, FLJ31314, C14orf41, c14_5527 |
| Ig Type | IgG |
| 交叉反应 | Human,Mouse,Rat |
| 验证活性 | 1. Western blot analysis of NUMB on A431 cells lysates using anti-NUMB antibody at 1/1,000 dilution. 2. Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-NUMB antibody. Counter stained with hematoxylin. 3. Immunohistochemical analysis of paraffin-embedded rat adrenal gland tissue using anti-NUMB antibody. Counter stained with hematoxylin. 4. Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-NUMB antibody. Counter stained with hematoxylin. 5. ICC staining NUMB in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 6. ICC staining NUMB in A431 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 7. ICC staining NUMB in NIH/3T3 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
| 应用 | |
| 推荐剂量 | WB: 1:1000-5000; IHC: 1:50-100; ICC: 1:50-200 |
| 抗体种类 | Monoclonal |
| 宿主来源 | Rabbit |
| 构建方式 | Recombinant Antibody |
| 纯化方式 | ProA affinity purified |
| 性状 | Liquid |
| 缓冲液 | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| 研究背景 | Neuronal cell fate decisions are directed in Drosophila by NUMB, a signaling adapter protein with two protein-protein interaction domains: a phosphotyrosine-binding domain and a proline-rich SH3-binding region (PRR). Mammalian NUMB homologs play a role in the determination of cell fates during development and bind with EPS15, LNX1, and NOTCH1. Conditional mouse mutants with deletion of NUMB in developing sensory ganglia show a reduction in axonal arborization in afferent fibers. Changes in cellular calcium homeostasis influences NUMB-dependent cell fate decisions during development of the nervous system. Chicken NUMB (c-NUMB) protein is localized to the basal cortex of mitotic neuroepithelial cells. |
| 偶联 | Unconjugated |
| 免疫原 | Recombinant Protein |
| Uniprot ID |
| 分子量 | Theoretical: 71 kDa. |
存储&运输
| 储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |
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比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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