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Anti-Nucleoside phosphorylase/PNP Antibody (1O563)

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Anti-Nucleoside phosphorylase/PNP Antibody (1O563)
产品编号 TMAY-02237
别名 purine nucleoside phosphorylase, PUNP, PRO1837, NP

Anti-Nucleoside phosphorylase/PNP Antibody (1O563) 是一种 Rabbit 抗体，靶向 Nucleoside phosphorylase/PNP。Anti-Nucleoside phosphorylase/PNP Antibody (1O563) 可用于 WB,ELISA,IP。

Anti-Nucleoside phosphorylase/PNP Antibody (1O563)

Anti-Nucleoside phosphorylase/PNP Antibody (1O563)

Rating icon 还可以
Anti-Nucleoside phosphorylase/PNP Antibody (1O563)
产品编号 TMAY-02237 别名 purine nucleoside phosphorylase, PUNP, PRO1837, NP

Anti-Nucleoside phosphorylase/PNP Antibody (1O563) 是一种 Rabbit 抗体，靶向 Nucleoside phosphorylase/PNP。Anti-Nucleoside phosphorylase/PNP Antibody (1O563) 可用于 WB,ELISA,IP。

规格价格库存数量
100 μL
¥ 2,279
5日内发货
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产品介绍

生物活性
产品描述
Anti-Nucleoside phosphorylase/PNP Antibody (1O563) is a Rabbit antibody targeting Nucleoside phosphorylase/PNP. Anti-Nucleoside phosphorylase/PNP Antibody (1O563) can be used in WB,ELISA,IP.
别名purine nucleoside phosphorylase, PUNP, PRO1837, NP
Ig Type
Rabbit IgG
克隆号
1O563
交叉反应
Human
验证活性
1. Anti-PNP3 rabbit monoclonal antibody at 1:500 dilution.
-Lane A: Jurkat Whole Cell Lysate.
-Lane B: K562 Whole Cell Lysate.
-Lane C: 293T Whole Cell lysate.
-Lysates/proteins at 30 μg per lane.
-Secondary
-Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.
-Developed using the Odyssey technique.
-Performed under reducing conditions.
-Predicted band size:32 kDa.
-Observed band size:32 kDa.
2. PNP-3 was immunoprecipitated using:
-Lane A:0.5 mg Jurkat Whole Cell Lysate.
-Lane B:0.5 mg K562 Whole Cell Lysate.
-2 µL anti-PNP-3 rabbit monoclonal antibody and 15 μl of 50 % Protein G agarose.
-Primary antibody:
-Anti-PNP-3 rabbit monoclonal antibody, at 1:100 dilution.
-Secondary antibody:
-Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution.
-Developed using the odyssey technique.
-Performed under reducing conditions.
-Predicted band size: 33 kDa.
-Observed band size: 33 kDa
应用
WB
ELISA
IP
推荐剂量
WB: 1:500-1:2000; ELISA: 1:5000-1:10000; IP: 1-4 μL/mg of lysate
抗体种类
Monoclonal
宿主来源Rabbit
构建方式This antibody was obtained from a rabbit immunized with purified, recombinant Human Purine nucleoside phosphorylase / PNP (rh Purine nucleoside phosphorylase / PNP; TMPY-02279; P00491; Met 1-Ser 289).
纯化方式Protein A
性状Liquid
缓冲液0.2 μm filtered solution in PBS
研究背景Purine nucleoside phosphorylase (PNP) is a purine-metabolizing enzyme that catalyzes the reversible phosphorolysis of 6-oxypurine (deoxy)nucleosides to their respective bases and (deoxy)ribose-1-phosphate. It is a key enzyme in the purine salvage pathway of mammalian cells. Purine nucleoside phosphorylase is a transferase that catalyzes the addition of phosphate and removal of a purine base from guanosine and similar nucleosides.PNP defects result in metabolic abnormalities and fatal T cell immunodeficiency. Purine nucleoside phosphorylase (PNP) is a target for leukemia, gout, and autoimmune disorders.
偶联与修饰
偶联
Unconjugated
抗原信息
免疫原
Recombinant Protein: Human Purine nucleoside phosphorylase / PNP protein (TMPY-02279)
抗原种属
Human
存储&运输
储存方式Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free.
运输方式Shipping with blue ice.

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关键词

PRO-1837PRO 1837

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