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Anti-Nucleoside phosphorylase/PNP Antibody (1O563)
还可以产品编号 TMAY-02237
Anti-Nucleoside phosphorylase/PNP Antibody (1O563) 是一种 Rabbit 抗体，靶向 Nucleoside phosphorylase/PNP。Anti-Nucleoside phosphorylase/PNP Antibody (1O563) 可用于 WB,ELISA,IP。
Anti-Nucleoside phosphorylase/PNP Antibody (1O563)
Anti-Nucleoside phosphorylase/PNP Antibody (1O563)
还可以产品编号 TMAY-02237
Anti-Nucleoside phosphorylase/PNP Antibody (1O563) 是一种 Rabbit 抗体，靶向 Nucleoside phosphorylase/PNP。Anti-Nucleoside phosphorylase/PNP Antibody (1O563) 可用于 WB,ELISA,IP。
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产品介绍
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偶联与修饰
抗原信息
| 产品描述 | Anti-Nucleoside phosphorylase/PNP Antibody (1O563) is a Rabbit antibody targeting Nucleoside phosphorylase/PNP. Anti-Nucleoside phosphorylase/PNP Antibody (1O563) can be used in WB,ELISA,IP. |
| Ig Type | Monoclonal Rabbit IgG |
| 克隆号 | 1O563 |
| 交叉反应 | Human |
| 验证活性 | 1. Anti-PNP3 rabbit monoclonal antibody at 1:500 dilution. -Lane A: Jurkat Whole Cell Lysate. -Lane B: K562 Whole Cell Lysate. -Lane C: 293T Whole Cell lysate. -Lysates/proteins at 30 μg per lane. -Secondary -Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. -Developed using the Odyssey technique. -Performed under reducing conditions. -Predicted band size:32 kDa. -Observed band size:32 kDa. 2. PNP-3 was immunoprecipitated using: -Lane A:0.5 mg Jurkat Whole Cell Lysate. -Lane B:0.5 mg K562 Whole Cell Lysate. -2 µL anti-PNP-3 rabbit monoclonal antibody and 15 μl of 50 % Protein G agarose. -Primary antibody: -Anti-PNP-3 rabbit monoclonal antibody, at 1:100 dilution. -Secondary antibody: -Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. -Developed using the odyssey technique. -Performed under reducing conditions. -Predicted band size: 33 kDa. -Observed band size: 33 kDa |
| 应用 | WB,ELISA,IP |
| 推荐剂量 | WB: 1:500-1:2000; ELISA: 1:5000-1:10000; IP: 1-4 μL/mg of lysate |
| 抗体种类 | Monoclonal |
| 宿主来源 | Rabbit |
| 构建方式 | This antibody was obtained from a rabbit immunized with purified, recombinant Human Purine nucleoside phosphorylase / PNP (rh Purine nucleoside phosphorylase / PNP; TMPY-02279; P00491; Met 1-Ser 289). |
| 纯化方式 | Protein A |
| 性状 | Liquid |
| 缓冲液 | 0.2 μm filtered solution in PBS |
| 研究背景 | Purine nucleoside phosphorylase (PNP) is a purine-metabolizing enzyme that catalyzes the reversible phosphorolysis of 6-oxypurine (deoxy)nucleosides to their respective bases and (deoxy)ribose-1-phosphate. It is a key enzyme in the purine salvage pathway of mammalian cells. Purine nucleoside phosphorylase is a transferase that catalyzes the addition of phosphate and removal of a purine base from guanosine and similar nucleosides.PNP defects result in metabolic abnormalities and fatal T cell immunodeficiency. Purine nucleoside phosphorylase (PNP) is a target for leukemia, gout, and autoimmune disorders. |
| 偶联 | Unconjugated |
| 免疫原 | Recombinant Human Purine nucleoside phosphorylase / PNP protein (TMPY-02279) |
| 抗原种属 | Human |
存储&运输
| 储存方式 | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| 运输方式 | Shipping with blue ice. |
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比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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