Anti-NPAS2 Polyclonal Antibody is a Rabbit antibody targeting NPAS2. Anti-NPAS2 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

IgG

Human,Rat (predicted:Mouse,Dog)

1. Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Neuronal PAS domain-containing protein 2; NPAS2) Polyclonal Antibody, Unconjugated (TMAB-01256) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 min and DAB staining.
2. Sample:
Lane 1: Human A549 cell lysates
Lane 2: Human HepG2 cell lysates
Lane 3: Human HeLa cell lysates
Lane 4: Human MCF-7 cell lysates
Primary: Anti-NPAS2 (TMAB-01256) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 92 kDa
Observed band size: 105 kDa

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human NPAS2

Human