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顶部产品介绍抗原信息化学信息储存和溶解信息计算器

Anti-Nkx2.5 Polyclonal Antibody

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货号 TMAB-09507

Anti-Nkx2.5 Polyclonal Antibody 是一种 Rabbit 抗体，靶向 Nkx2.5。Anti-Nkx2.5 Polyclonal Antibody 可用于 IHC-P,IHC-Fr,IF。

Anti-Nkx2.5 Polyclonal Antibody

Anti-Nkx2.5 Polyclonal Antibody

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Rating icon 还可以
货号 TMAB-09507

Anti-Nkx2.5 Polyclonal Antibody 是一种 Rabbit 抗体，靶向 Nkx2.5。Anti-Nkx2.5 Polyclonal Antibody 可用于 IHC-P,IHC-Fr,IF。

规格价格库存数量
50 μL
¥ 1,160
5日内发货
100 μL
¥ 1,975
5日内发货
200 μL
¥ 2,795
5日内发货
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产品介绍

生物活性
产品描述
Anti-Nkx2.5 Polyclonal Antibody is a Rabbit antibody targeting Nkx2.5. Anti-Nkx2.5 Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF.
Ig Type
IgG
反应种属
Human,Rat (predicted:Mouse,Dog,Pig,Cow,Rabbit)
验证活性
1. Tissue/cell: Rat heart; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37℃ for 20 min;
Incubation: Anti-Nkx2.5/Cardiac-specific homeobox 1 Polyclonal Antibody, Unconjugated (TMAB-09507) 1: 200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining
2. Paraformaldehyde-fixed, paraffin embedded (human heart); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Nkx2.5) Polyclonal Antibody, Unconjugated (TMAB-09507) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.
应用IHC-PIHC-FrIF
推荐剂量
IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500
抗体种类
Polyclonal
宿主来源Rabbit
亚细胞定位Nuclear
构建方式Polyclonal Antibody
纯化方式Protein A purified
性状Liquid
缓冲液0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
浓度1mg/ml
研究背景In humans, mutations of the gene encoding the homeobox transcription factor Nkx2.5 result in electrical conduction defects and morphological abnormalities of the heart (Dupays et al., 2005). In the heart, Nkx2.5 is expressed in both the myocardium and the endocardium. Differentiation of embryonic stem cells to Nkx2.5-positive cardiomyocytes is facilitated by Wnt11.
抗原信息
免疫原
KLH conjugated synthetic peptide: human Nkx2.5
抗原种属
Human
基因名称
NKX2-5
基因ID
1482
蛋白名称
Homeobox protein Nkx-2.5
Uniprot ID
P52952
化学信息
分子量Theoretical: 36 kDa.
储存&溶解度
储存方式Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
运输方式Shipping with blue ice.

计算器

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