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Anti-NFKBIA Antibody (1J77) 是一种 Rabbit 抗体,靶向 NFKBIA。Anti-NFKBIA Antibody (1J77) 可用于 WB,ICC/IF,IHC,IP。
Anti-NFKBIA Antibody (1J77) 是一种 Rabbit 抗体,靶向 NFKBIA。Anti-NFKBIA Antibody (1J77) 可用于 WB,ICC/IF,IHC,IP。
规格 | 价格 | 库存 | 数量 |
---|---|---|---|
50 μL | ¥ 1,495 | 5日内发货 | |
100 μL | ¥ 2,485 | 5日内发货 |
产品描述 | Anti-NFKBIA Antibody (1J77) is a Rabbit antibody targeting NFKBIA. Anti-NFKBIA Antibody (1J77) can be used in WB,ICC/IF,IHC,IP. |
别名 | nuclear factor of κ light polypeptide gene enhancer in B-cells inhibitor, alpha, nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, α, nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha, NFKBI, MAD-3, IKBA, IkB α |
Ig Type | IgG |
交叉反应 | Human,Mouse,Rat |
验证活性 | 1. Western blot analysis of IKB alpha on different lysates using anti-IKB alpha antibody at 1/1,000 dilution. Positive control: Lane 1: PC-12, Lane 2: NIH/3T3, Lane 3: Hela. 2. Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-IKB alpha antibody. Counter stained with hematoxylin. 3. Immunohistochemical analysis of paraffin-embedded mouse stomach tissue using anti-IKB alpha antibody. Counter stained with hematoxylin. 4. Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-IKB alpha antibody. Counter stained with hematoxylin. 5. Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-IKB alpha antibody. Counter stained with hematoxylin. 6. ICC staining IKB alpha in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 7. ICC staining IKB alpha in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 8. ICC staining IKB alpha in SHG-44 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
应用 | |
推荐剂量 | WB: 1:1000-5000; IHC: 1:50-200; ICC/IF: 1:50-200 |
抗体种类 | Monoclonal |
宿主来源 | Rabbit |
构建方式 | Recombinant Antibody |
纯化方式 | ProA affinity purified |
性状 | Liquid |
缓冲液 | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
研究背景 | NFKB1 or NFKB2 is bound to REL, RELA, or RELB to form the NFKB complex. The NFKB complex is inhibited by I-kappa-B proteins, which inactivate NF-kappa-B by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime. |
偶联 | Unconjugated |
免疫原 | Recombinant Protein |
Uniprot ID |
分子量 | Theoretical: 34 kDa. |
储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
运输方式 | Shipping with blue ice. |
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