Anti-NF-kB p65 Polyclonal Antibody 6 is a Rabbit antibody targeting NF-kB p65. Anti-NF-kB p65 Polyclonal Antibody 6 can be used in WB,FCM.

IgG

Human,Mouse (predicted:Rat,Dog,Pig,Cow,Horse,Sheep,GuineaPig)

1. Sample:
Hcclm3 (Human) Cell Lysate at 40 μg
Lung (Mouse) Lysate at 40 μg
Primary: Anti-NFKB p65 (acetyl-K310) (TMAB-01232) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 61 kDa
Observed band size: 61 kDa
2. Blank control: HL-60. Primary Antibody (green line): Rabbit Anti-NFKB p65 (acetyl K310) antibody (TMAB-01232)
Dilution: 1 μg/10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody: Goat anti-rabbit IgG-FITC
Dilution: 1 μg/test.
Protocol
The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at-20°C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature.

WB: 1:500-2000; FCM: 1ug/Test

Polyclonal

KLH conjugated Synthesised acetylpeptide: human NFKBp65 around the acetylation site of Lys310

Human

RelA (p65),NFkB pathway,Inflammatory mediators,RelA (p65),NFkB pathway,Obesity,Host Immune Response,Host Virus Interaction,Alzheimer's disease,NFkB Pathway