Anti-Neurogranin Polyclonal Antibody is a Rabbit antibody targeting Neurogranin. Anti-Neurogranin Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF.

IgG

Mouse,Rat (predicted:Human,Dog,Pig,Cow,Horse,Rabbit)

1. Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37℃ for 20 min;
Incubation: Anti-Neurogranin Polyclonal Antibody, Unconjugated (TMAB-09393) 1: 200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining
2. Positive sample: Paraformaldehyde-fixed, paraffin embedded (rat brian);
Negative sample: Paraformaldehyde-fixed, paraffin embedded (rat liver);
Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Neurogranin) Polyclonal Antibody, Unconjugated (TMAB-09393) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.
3. Positive sample: Paraformaldehyde-fixed, paraffin embedded (mouse brain);
Negative sample: Paraformaldehyde-fixed, paraffin embedded (mouse heart);
Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Neurogranin) Polyclonal Antibody, Unconjugated (TMAB-09393) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.

IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human NG/Neurogranin

Human

NRGN

Neurogranin

Acts as a "third messenger" substrate of protein kinase C-mediated molecular cascades during synaptic development and remodeling. Binds to calmodulin in the absence of calcium.