Anti-Neurofascin Polyclonal Antibody is a Rabbit antibody targeting Neurofascin. Anti-Neurofascin Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

IgG

Mouse,Rat (predicted:Human,Chicken,Dog,Cow,Horse)

1. Tissue/cell: rat spinal cord tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37℃ for 20 min;
Incubation: Anti-Neurofascin Polyclonal Antibody, Unconjugated (TMAB-09388) 1: 200, overnight at 4℃, followed by conjugation to the secondary antibody and DAB staining
2. Tissue/cell: Rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37℃ for 20 min;
Incubation: Anti-Neurofascin Polyclonal Antibody, Unconjugated (TMAB-09388) 1:500, overnight at 4℃, followed by conjugation to the secondary antibody and DAB staining
3. Sample: Cerebellum (Mouse) Lysate at 30 μg
Primary: Anti-Neurofascin Polyclonal (TMAB-09388) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 132/150 kD
Observed band size: 136 kD
4. Sample:
Lane 1: Cerebrum (Mouse) Lysate at 40 μg
Lane 2: Cerebellum (Mouse) Lysate at 40 μg
Lane 3: Cerebrum (Rat) Lysate at 40 μg
Lane 4: Cerebellum (Rat) Lysate at 40 μg
Primary: Anti-Neurofascin (TMAB-09388) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 132 kD
Observed band size: 132 kD
5. Sample: Cerebral cortex (Mouse) Lysate at 40 μg
Primary: Anti-Neurofascin (TMAB-09388) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 132/150 kD
Observed band size: 150 kD

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human Neurofascin-155

Human

NFASC

Neurofascin

Cell adhesion, ankyrin-binding protein which may be involved in neurite extension, axonal guidance, synaptogenesis, myelination and neuron-glial cell interactions.