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Anti-NeuroD1 Antibody (6V209)
还可以产品编号 TMAH-00812
别名 Neurogenic differentiation factor 1, NeuroD 1, NeuroD, NEUROD, Class A basic helix-loop-helix protein 3, BHLHA3
Anti-NeuroD1 Antibody (6V209) 是一种抗体，靶向 NeuroD1。Anti-NeuroD1 Antibody (6V209) 可用于 ELISA, IF, FC。
Anti-NeuroD1 Antibody (6V209)
Anti-NeuroD1 Antibody (6V209)
还可以产品编号 TMAH-00812 别名 Neurogenic differentiation factor 1, NeuroD 1, NeuroD, NEUROD, Class A basic helix-loop-helix protein 3, BHLHA3
Anti-NeuroD1 Antibody (6V209) 是一种抗体，靶向 NeuroD1。Anti-NeuroD1 Antibody (6V209) 可用于 ELISA, IF, FC。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 50 μL | ¥ 1,310 | 5日内发货 | |
| 100 μL | ¥ 2,180 | 5日内发货 |
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产品介绍
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抗原信息
| 产品描述 | Anti-NeuroD1 Antibody (6V209) is an antibody targeting NeuroD1. Anti-NeuroD1 Antibody (6V209) can be used in ELISA, IF, FC. |
| 别名 | Neurogenic differentiation factor 1, NeuroD 1, NeuroD, NEUROD, Class A basic helix-loop-helix protein 3, BHLHA3 |
| Ig Type | Rabbit IgG |
| 克隆号 | 6V209 |
| 交叉反应 | Human |
| 验证活性 | 1. Immunofluorescence staining of A549 with TMAH-00812 at 1:60, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 522-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). 2. Overlay Peak curve showing SH-SY5Y cells stained with TMAH-00812 (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1µg/1*10^6 cells) for 45min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4°C.Control antibody (green line) was rabbit IgG (1µg/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed. |
| 应用 | ELISA, IF, FC |
| 推荐剂量 | IF:1:50-1:200; FC:1:50-1:200. |
| 抗体种类 | Monoclonal |
| 亚细胞定位 | Cytoplasm. Nucleus. |
| 构建方式 | Recombinant Antibody |
| 纯化方式 | Affinity-chromatography |
| 性状 | Liquid |
| 缓冲液 | Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| 研究背景 | Acts as a transcriptional activator: mediates transcriptional activation by binding to E box-containing promoter consensus core sequences 5'-CANNTG-3'. Associates with the p300/CBP transcription coactivator complex to stimulate transcription of the secretin gene as well as the gene encoding the cyclin-dependent kinase inhibitor CDKN1A. Contributes to the regulation of several cell differentiation pathways, like those that promote the formation of early retinal ganglion cells, inner ear sensory neurons, granule cells forming either the cerebellum or the dentate gyrus cell layer of the hippocampus, endocrine islet cells of the pancreas and enteroendocrine cells of the small intestine. Together with PAX6 or SIX3, is required for the regulation of amacrine cell fate specification. Also required for dendrite morphogenesis and maintenance in the cerebellar cortex. Associates with chromatin to enhancer regulatory elements in genes encoding key transcriptional regulators of neurogenesis. |
| 偶联 | Unconjugated |
| 免疫原 | A synthetic peptide: Human NEUROD1 |
| 抗原种属 | Human |
| 基因ID | 4760 |
| Uniprot ID | |
| 研究领域 | Epigenetics and Nuclear Signaling, Neuroscience, Cancer, Cardiovascular, Metabolism |
存储&运输
| 储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |
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比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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