Anti-Netrin-1 Polyclonal Antibody is a Rabbit antibody targeting Netrin-1. Anti-Netrin-1 Polyclonal Antibody can be used in ICC/IF,IF,IHC-Fr,IHC-P.

IgG

Human,Rat (predicted:Mouse,Chicken,Dog,Pig,Cow)

1. Paraformaldehyde-fixed, paraffin embedded (Rat adrenal gland); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Netrin 1) Polyclonal Antibody, Unconjugated (TMAB-01217) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 min and DAB staining.
2. Paraformaldehyde-fixed, paraffin embedded (rat stomach tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Netrin 1) Polyclonal Antibody, Unconjugated (TMAB-01217) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 min and DAB staining.
3. SH-SY5Y cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Antibody incubation with (Netrin 1) polyclonal Antibody, Unconjugated (TMAB-01217) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nucleus.

IHC-P: 1:100-500; IHC-Fr: 1:100-500; ICC/IF: 1:100; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human NtN1

Human

NTN1

Netrin-1

Axonal Guidance Proteins,Neurogenesis,Other ECM Proteins

Netrins control guidance of CNS commissural axons and peripheral motor axons. Its association with either DCC or some UNC5 receptors will lead to axon attraction or repulsion, respectively. It also serve as a survival factor via its association with its receptors which prevent the initiation of apoptosis. Involved in tumorigenesis by regulating apoptosis.