Anti-NET1 Polyclonal Antibody is a Rabbit antibody targeting NET1. Anti-NET1 Polyclonal Antibody can be used in ICC/IF,IF,IHC-Fr,IHC-P,WB.

IgG

Human,Mouse,Rat (predicted:Chicken,Dog,Pig,Cow,Horse,Rabbit,Sheep)

1. Tissue/cell: mouse brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-NET1 Polyclonal Antibody, Unconjugated (TMAB-01216) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
2. Sample:
Jurkat (Human) Cell Lysate at 30 μg
Skin (Mouse) Lysate at 40 μg
Skin (Rat) Lysate at 40 μg
Primary: Anti-NET1 (TMAB-01216) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 65 kDa
Observed band size: 65 kDa
3. Sample:
Lane 1: Mouse Cerebrum tissue lysates
Lane 2: Mouse Cerebellum tissue lysates
Lane 3: Mouse Heart tissue lysates
Lane 4: Rat Cerebrum tissue lysates
Lane 5: Rat Cerebellum tissue lysates
Lane 6: Rat Stomach tissue lysates
Lane 7: Human K562 cell lysates
Lane 8: Human A431 cell lysates
Lane 9: Human HepG2 cell lysates
Lane 10: Human MCF-7 cell lysates
Primary: Anti-NET1 (TMAB-01216) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 65 kDa
Observed band size: 62 kDa
4. HepG2 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Antibody incubation with (NET1) polyclonal Antibody, Unconjugated (TMAB-01216) 1:25, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nucleus.

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; ICC/IF: 1:25; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human NET1

Human

NET1

Neuroepithelial cell-transforming gene 1 protein

Ras family,Other factors,Ras Family

Acts as guanine nucleotide exchange factor (GEF) for RhoA GTPase. May be involved in activation of the SAPK/JNK pathway Stimulates genotoxic stress-induced RHOB activity in breast cancer cells leading to their cell death.