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Anti-NEFH Polyclonal Antibody

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货号 TMAB-01211

别名 NF-H, NFH, Neurofilament triplet H protein, Neurofilament heavy polypeptide, NEFH, KIAA0845, 200 kDa neurofilament protein

Anti-NEFH Polyclonal Antibody 是一种 Rabbit 抗体，靶向 NEFH。Anti-NEFH Polyclonal Antibody 可用于 IF,IHC-Fr,IHC-P。

Anti-NEFH Polyclonal Antibody

Anti-NEFH Polyclonal Antibody

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货号 TMAB-01211 别名 NF-H, NFH, Neurofilament triplet H protein, Neurofilament heavy polypeptide, NEFH, KIAA0845, 200 kDa neurofilament protein

Anti-NEFH Polyclonal Antibody 是一种 Rabbit 抗体，靶向 NEFH。Anti-NEFH Polyclonal Antibody 可用于 IF,IHC-Fr,IHC-P。

规格价格库存数量
50 μL
¥ 1,160
5日内发货
100 μL
¥ 1,970
5日内发货
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产品描述
Anti-NEFH Polyclonal Antibody is a Rabbit antibody targeting NEFH. Anti-NEFH Polyclonal Antibody can be used in IF,IHC-Fr,IHC-P.
别名NF-H, NFH, Neurofilament triplet H protein, Neurofilament heavy polypeptide, NEFH, KIAA0845, 200 kDa neurofilament protein
Ig Type
IgG
反应种属
Human,Mouse,Rat
验证活性
1. Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (NF-H) Polyclonal Antibody, Unconjugated (TMAB-01211) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
2. Paraformaldehyde-fixed, paraffin embedded (rat cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (NF-H) Polyclonal Antibody, Unconjugated (TMAB-01211) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded (mouse cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (NF-H) Polyclonal Antibody, Unconjugated (TMAB-01211) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
verifiedActivityverifiedActivityverifiedActivity
应用IFIHC-FrIHC-P
推荐剂量
IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500
抗体种类
Polyclonal
宿主来源Rabbit
构建方式Polyclonal Antibody
纯化方式Protein A purified
性状Liquid
缓冲液0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
浓度1 mg/mL
研究背景Neurofilaments can be defined as the intermediate or 10nm filaments found in specifically in neuronal cells. When visualised using an electron microscope, neurofilaments appear as 10nm diameter fibres of indeterminate length that generally have fine wispy protrusions from their sides. They are particularly abundant in axons of large projection neurons. They probably function to provide structural support for neurons and their synapses and to support the large axon diameters required for rapid conduction of impulses down axons. Neurofilaments are composed of a mixture of subunits, which usually includes the three neurofilament triplet proteins neurofilament light (NFL), neurofilament medium (NFM) and neurofilament heavy (NFH). Neurofilaments may also include smaller amounts of peripherin, alpha internexin, nestin and in some cases vimentin. Antibodies to the various neurofilament subunits are very useful cell type markers since the proteins are among the most abundant of the nervous system, are expressed only in neurons, and are biochemically very stable. Some studies have shown that levels of neurofilament heavy and neurofilament light are elevated in patients with Alzheimer's disease, frontotemporal lobe dementia, and vascular dementia.
抗原信息
免疫原
KLH conjugated synthetic peptide: human NF-H
抗原种属
Human
基因名称
NEFH
基因ID
4744
蛋白名称
Neurofilament heavy polypeptide
Uniprot ID
P12036
研究领域
Intermediate Filaments,Axon marker,Alzheimer's disease,Neurodegenerative disease,Neurofilaments
功能
Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. NF-H has an important function in mature axons that is not subserved by the two smaller NF proteins.
化学信息
分子量Theoretical: 118 kDa. Actual: 200 kDa.
储存&溶解度
储存方式Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
运输方式Shipping with blue ice.
存储store at low temperature | store at -20°C

计算器

  • 稀释 计算器

关键词

KIAA-0845
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