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Anti-NCOA4 Polyclonal Antibody 是一种 Rabbit 抗体,靶向 NCOA4。Anti-NCOA4 Polyclonal Antibody 可用于 WB,IHC-P,IHC-Fr,IF。
Anti-NCOA4 Polyclonal Antibody 是一种 Rabbit 抗体,靶向 NCOA4。Anti-NCOA4 Polyclonal Antibody 可用于 WB,IHC-P,IHC-Fr,IF。
规格 | 价格 | 库存 | 数量 |
---|---|---|---|
50 μL | ¥ 1,160 | 5日内发货 | |
100 μL | ¥ 1,975 | 5日内发货 | |
200 μL | ¥ 2,795 | 5日内发货 |
产品描述 | Anti-NCOA4 Polyclonal Antibody is a Rabbit antibody targeting NCOA4. Anti-NCOA4 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF. |
Ig Type | IgG |
交叉反应 | Human (predicted:Mouse,Rat,Pig,Cow,Horse,Sheep) |
验证活性 | 1. Paraformaldehyde-fixed, paraffin embedded (Human duodenum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (NCOA4) Polyclonal Antibody, Unconjugated (TMAB-01206) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 2. Sample: Lane 1: HT1080 (Human) Cell Lysate at 30 μg Lane 2: HepG2 (Human) Cell Lysate at 30 μg Lane 3: U937 (Human) Cell Lysate at 30 μg Primary: Anti-NCOA4 (TMAB-01206) at 1/1000 dilution Anti-beta-Actin at 1/2000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 70 kDa Observed band size: 85 kDa |
应用 | |
推荐剂量 | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
抗体种类 | Polyclonal |
宿主来源 | Rabbit |
构建方式 | Hybridoma Polyclonal Antibody |
纯化方式 | Protein A purified |
性状 | Liquid |
缓冲液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
研究背景 | This gene encodes an androgen receptor coactivator. The encoded protein interacts with the androgen receptor in a ligand-dependent manner to enhance its transcriptional activity. Chromosomal translocations between this gene and the ret tyrosine kinase gene, also located on chromosome 10, have been associated with papillary thyroid carcinoma. Alternatively spliced transcript variants have been described. Pseudogenes are present on chromosomes 4, 5, 10, and 14. [provided by RefSeq, Feb 2009] |
免疫原 | KLH conjugated synthetic peptide: human NCOA4 |
抗原种属 | Human |
基因ID | |
Uniprot ID | |
研究领域 | Co-activators/co-repressors,Co-activators/co-repressors |
储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
运输方式 | Shipping with blue ice. |
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