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Anti-NANOG Antibody (6Z745)
还可以产品编号 TMAH-00804
Anti-NANOG Antibody (6Z745) 是一种抗体，靶向 NANOG。Anti-NANOG Antibody (6Z745) 可用于 ELISA, FC。
Anti-NANOG Antibody (6Z745)
Anti-NANOG Antibody (6Z745)
还可以产品编号 TMAH-00804
Anti-NANOG Antibody (6Z745) 是一种抗体，靶向 NANOG。Anti-NANOG Antibody (6Z745) 可用于 ELISA, FC。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 50 μL | ¥ 1,305 | 5日内发货 | |
| 100 μL | ¥ 2,185 | 5日内发货 |
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产品介绍
生物活性
偶联与修饰
抗原信息
| 产品描述 | Anti-NANOG Antibody (6Z745) is an antibody targeting NANOG. Anti-NANOG Antibody (6Z745) can be used in ELISA, FC. |
| Ig Type | Rabbit IgG |
| 克隆号 | 6Z745 |
| 交叉反应 | Human |
| 验证活性 | Overlay Peak curve showing Hela cells stained with TMAH-00804 (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1µg/1*10^6 cells) for 45min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4°C.Control antibody (green line) was rabbit IgG (1µg/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed. |
| 应用 | ELISA, FC |
| 推荐剂量 | FC:1:50-1:200. |
| 抗体种类 | Monoclonal |
| 亚细胞定位 | Nucleus. |
| 构建方式 | Recombinant Antibody |
| 纯化方式 | Affinity-chromatography |
| 性状 | Liquid |
| 缓冲液 | Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| 研究背景 | Transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes. Acts as a transcriptional activator or repressor. Binds optimally to the DNA consensus sequence 5'-TAAT[GT][GT]-3' or 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3'. Binds to the POU5F1/OCT4 promoter. Able to autorepress its expression in differentiating (ES) cells: binds to its own promoter following interaction with ZNF281/ZFP281, leading to recruitment of the NuRD complex and subsequent repression of expression. When overexpressed, promotes cells to enter into S phase and proliferation. |
| 偶联 | Unconjugated |
| 免疫原 | A synthetic peptide: Human NANOG |
| 抗原种属 | Human |
| 基因ID | 79923 |
| Uniprot ID | |
| 研究领域 | Epigenetics and Nuclear Signaling, Cancer, Developmental biology, Stem cells |
存储&运输
| 储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |
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比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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