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Anti-NANOG Antibody (6Z745)
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产品编号 TMAH-00804
Anti-NANOG Antibody (6Z745) 是一种抗体，靶向 NANOG。Anti-NANOG Antibody (6Z745) 可用于 ELISA, FCM。
Anti-NANOG Antibody (6Z745)
Anti-NANOG Antibody (6Z745)
还可以产品编号 TMAH-00804
Anti-NANOG Antibody (6Z745) 是一种抗体，靶向 NANOG。Anti-NANOG Antibody (6Z745) 可用于 ELISA, FCM。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 50 μL | ¥ 1,305 | 5日内发货 | |
| 100 μL | ¥ 2,185 | 5日内发货 |
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产品介绍
生物活性
偶联与修饰
抗原信息
| 产品描述 | Anti-NANOG Antibody (6Z745) is an antibody targeting NANOG. Anti-NANOG Antibody (6Z745) can be used in ELISA, FCM. |
| Ig Type | Rabbit IgG |
| 克隆号 | 6Z745 |
| 交叉反应 | Human |
| 验证活性 | Overlay Peak curve showing Hela cells stained with TMAH-00804 (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1µg/1*10^6 cells) for 45min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4°C.Control antibody (green line) was rabbit IgG (1µg/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed. |
| 应用 | |
| 推荐剂量 | FCM:1:50-1:200. |
| 抗体种类 | Monoclonal |
| 亚细胞定位 | Nucleus. |
| 组织特异性 | Expressed in testicular carcinoma and derived germ cell tumors (at protein level). Expressed in fetal gonads, ovary and testis. Also expressed in ovary teratocarcinoma cell line and testicular embryonic carcinoma. Not expressed in many somatic organs and |
| 构建方式 | Recombinant Antibody |
| 纯化方式 | Affinity-chromatography |
| 性状 | Liquid |
| 缓冲液 | Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| 研究背景 | Transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes. Acts as a transcriptional activator or repressor. Binds optimally to the DNA consensus sequence 5'-TAAT[GT][GT]-3' or 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3'. Binds to the POU5F1/OCT4 promoter. Able to autorepress its expression in differentiating (ES) cells: binds to its own promoter following interaction with ZNF281/ZFP281, leading to recruitment of the NuRD complex and subsequent repression of expression. When overexpressed, promotes cells to enter into S phase and proliferation. |
| 偶联 | Unconjugated |
| 免疫原 | A synthetic peptide: Human NANOG |
| 抗原种属 | Human |
| 基因名称 | NANOG |
| 基因ID | |
| Uniprot ID | |
| 研究领域 | Epigenetics and Nuclear Signaling, Cancer, Developmental biology, Stem cells |
存储&运输
| 储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |
计算器
比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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