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Anti-MYH7 Polyclonal Antibody 2 是一种 Rabbit 抗体,靶向 MYH7。Anti-MYH7 Polyclonal Antibody 2 可用于 IHC-P,IHC-Fr,IF。
Anti-MYH7 Polyclonal Antibody 2 是一种 Rabbit 抗体,靶向 MYH7。Anti-MYH7 Polyclonal Antibody 2 可用于 IHC-P,IHC-Fr,IF。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 50 μL | ¥ 1,160 | 5日内发货 | |
| 100 μL | ¥ 1,965 | 5日内发货 | |
| 200 μL | ¥ 2,785 | 5日内发货 |
| 产品描述 | Anti-MYH7 Polyclonal Antibody 2 is a Rabbit antibody targeting MYH7. Anti-MYH7 Polyclonal Antibody 2 can be used in IHC-P,IHC-Fr,IF. |
| Ig Type | IgG |
| 反应种属 | Mouse,Rat (predicted:Human,Dog,Sheep) |
| 验证活性 | 1. Paraformaldehyde-fixed, paraffin embedded (rat skeletal muscle); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (MYH7) Polyclonal Antibody, Unconjugated (TMAB-09151) at 1: 200 overnight at 4°C, followed by a conjugated Goat Anti-Rabbit IgG antibody for 90 minutes, and DAPI for nuclei staining. 2. Paraformaldehyde-fixed, paraffin embedded (rat skeletal muscle); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (MYH7) Polyclonal Antibody, Unconjugated (TMAB-09151) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining. 3. Paraformaldehyde-fixed, paraffin embedded (mouse skeletal muscle); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (MYH7) Polyclonal Antibody, Unconjugated (TMAB-09151) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining. 4. Paraformaldehyde-fixed, paraffin embedded (rat heart); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (MYH7B) Polyclonal Antibody, Unconjugated (TMAB-09151) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining. |
| 应用 | IHC-PIHC-FrIF |
| 推荐剂量 | IHC-P: 1:400-800; IHC-Fr: 1:400-800; IF: 1:100-500 |
| 抗体种类 | Polyclonal |
| 宿主来源 | Rabbit |
| 亚细胞定位 | Cytoplasm, myofibril. Note=Thick filaments of the myofibrils. |
| 组织特异性 | Both wild type and variant Gln-403 are detected in skeletal muscle (at protein level). |
| 构建方式 | Polyclonal Antibody |
| 纯化方式 | Protein A purified |
| 性状 | Liquid |
| 缓冲液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 浓度 | 1mg/ml |
| 研究背景 | Myosin heavy chains are ubiquitous Actin-based motor proteins that convert the chemical energy derived from ATP hydrolysis into the mechanical energy that drives diverse motile processes in eukaryotic cells, including cytokinesis, vesicular transport and cellular locomotion. Muscle myosin is a heterohexamer consisting of two myosin heavy chains and two associated nonidentical pairs of myosin light chains. The seven myosin heavy chain isoforms that predominate in mammalian skeletal muscles include two developmental isoforms, MHC-embryonic (MYH3) and MHC-perinatal (MYH8); three adult skeletal muscle isoforms, MHC IIa (MYH2), MHC IIb (MYH4) and MHC IIx/d (MYH1); and MHC-∫/slow (MYH7 or MHC-∫), which is also expressed in cardiac muscle. Research indicates that mutations of the MYH7 gene causes hypertrophic cardiomyopathy. |
| 免疫原 | KLH conjugated synthetic peptide: human MYH7 |
| 抗原种属 | Human |
| 基因名称 | MYH7 |
| 基因ID | |
| 蛋白名称 | Myosin-7 |
| Uniprot ID | |
| 功能 | Myosins are actin-based motor molecules with ATPase activity essential for muscle contraction. Forms regular bipolar thick filaments that, together with actin thin filaments, constitute the fundamental contractile unit of skeletal and cardiac muscle. |
| 分子量 | Theoretical: 213 kDa. |
| 储存方式 | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |