购物车
- 全部删除
您的购物车当前为空
Anti-MYF5 Polyclonal Antibody
还可以
产品编号 TMAB-01193
Anti-MYF5 Polyclonal Antibody 是一种 Rabbit 抗体，靶向 MYF5。Anti-MYF5 Polyclonal Antibody 可用于 WB,IHC-P,IHC-Fr,IF。
Anti-MYF5 Polyclonal Antibody
Anti-MYF5 Polyclonal Antibody
还可以产品编号 TMAB-01193
Anti-MYF5 Polyclonal Antibody 是一种 Rabbit 抗体，靶向 MYF5。Anti-MYF5 Polyclonal Antibody 可用于 WB,IHC-P,IHC-Fr,IF。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 50 μL | ¥ 1,160 | 5日内发货 | |
| 100 μL | ¥ 1,960 | 5日内发货 | |
| 200 μL | ¥ 2,785 | 5日内发货 |
大包装 & 定制
加入购物车
TargetMol 的所有产品仅用作科学研究或药证申报，不能被用于人体，我们不向个人提供产品和服务。请您遵守承诺用途，不得违反法律法规规定用于任何其他用途。
联系我们获取更多批次信息
资源下载
产品介绍
生物活性
抗原信息
| 产品描述 | Anti-MYF5 Polyclonal Antibody is a Rabbit antibody targeting MYF5. Anti-MYF5 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF. |
| Ig Type | IgG |
| 交叉反应 | Mouse,Rat (predicted:Human,Chicken,Pig,Cow,Horse,Rabbit,Sheep) |
| 验证活性 | 1. Paraformaldehyde-fixed, paraffin embedded (mouse embryos tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (MYF5) Polyclonal Antibody, Unconjugated (TMAB-01193) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 2. Sample: Lane 1: Muscle (Mouse) Lysate at 40 μg Lane 2: Tongue (Mouse) Lysate at 40 μg Lane 3: Muscle (Rat) Lysate at 40 μg Lane 4: Tongue (Rat) Lysate at 40 μg Primary: Anti-MYF5 (TMAB-01193) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 28 kDa Observed band size: 28 kDa 3. Paraformaldehyde-fixed, paraffin embedded (rat skeletal muscle); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (MYF5) Polyclonal Antibody, Unconjugated (TMAB-01193) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 4. Paraformaldehyde-fixed, paraffin embedded (Rat tongue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (MYF5) Polyclonal Antibody, Unconjugated (TMAB-01193) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
| 应用 | |
| 推荐剂量 | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:50-200 |
| 抗体种类 | Polyclonal |
| 宿主来源 | Rabbit |
| 亚细胞定位 | Nucleus. |
| 构建方式 | Hybridoma Polyclonal Antibody |
| 纯化方式 | Protein A purified |
| 性状 | Liquid |
| 缓冲液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究背景 | Differentiation of myogenic cells is regulated by multiple positively and negatively acting factors. One well characterized family of helix-loop-helix (HLH) proteins known to play an important role in the regulation of muscle cell development include Myo D, myogenin, Myf-5 and Myf-6 (also designated MRF-4 or herculin). Of interest, most muscle cells express either Myo D or Myf-5 in the committed state, but when induced to differentiate, all turn on expression of myogenin. Myo D transcription factors form heterodimers with products of a more widely expressed family of bHLH genes, the E family, which consists of at least three distinct genes: E2A, IF2 and HEB. Myo D-E heterodimers bind avidly to consensus (CANNTG) E box target sites that are functionally important elements in the upstream regulatory sequences of many muscle-specific terminal differentiation genes. |
| 免疫原 | KLH conjugated synthetic peptide: human MYF5 |
| 抗原种属 | Human |
| 基因ID | |
| Uniprot ID | |
| 研究领域 | Myogenesis,Transcription Factors,Helix-Turn-Helix,Muscle |
存储&运输
| 储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |
计算器
比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
SCI 文献
评论列表
预览
Related Tags: buy Anti-MYF5 Polyclonal Antibody | purchase Anti-MYF5 Polyclonal Antibody | Anti-MYF5 Polyclonal Antibody cost | order Anti-MYF5 Polyclonal Antibody
嗨！有任何问题？点我咨询
评论内容