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Anti-Myc Tag Antibody (6S669)
还可以产品编号 TMAY-00032
Anti-Myc Tag Antibody (6S669) 是一种 Mouse 抗体，靶向 Myc Tag。Anti-Myc Tag Antibody (6S669) 可用于 WB,IP。
Anti-Myc Tag Antibody (6S669)
Anti-Myc Tag Antibody (6S669)
还可以产品编号 TMAY-00032
Anti-Myc Tag Antibody (6S669) 是一种 Mouse 抗体，靶向 Myc Tag。Anti-Myc Tag Antibody (6S669) 可用于 WB,IP。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 20 μL | ¥ 780 | 5日内发货 | |
| 100 μL | ¥ 1,580 | 5日内发货 | |
| 500 μL | ¥ 3,990 | 5日内发货 |
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产品介绍
生物活性
偶联与修饰
抗原信息
| 产品描述 | Anti-Myc Tag Antibody (6S669) is a Mouse antibody targeting Myc Tag. Anti-Myc Tag Antibody (6S669) can be used in WB,IP. |
| Ig Type | Monoclonal Mouse IgG1 |
| 克隆号 | 6S669 |
| 交叉反应 | other |
| 验证活性 | 1. Anti-Myc Tag mouse monoclonal antibody at 1:5000 dilution. -Lane A: GST-myc(Recombinant protein) (5ng) -Lane B: GST-myc(Recombinant protein) (1ng) -Lane C: myc-GST(Recombinant protein) (100ng) -Lane D: myc-GST(Recombinant protein) (20ng) -Secondary -Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution. -Developed using the Odyssey technique. -Performed under reducing conditions. 2. Anti-Myc Tag mouse monoclonal antibody at 1:5000 dilution. -Lane A: HA-mFABP4-myc transfected 293 cell lysate (10ug) -Lane B: myc-ARG1-HA transfected 293 cell lysate (5ug) -Lane C: HA-ARG1-myc transfected 293 cell lysate (10ug) -Secondary -Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution. -Developed using the Odyssey technique. -Performed under reducing conditions. 3. MYC was immunoprecipitated using: -Lane A:0.5 mg HA-ARG1-myc Whole Cell Lysate. -Lane B:0.5 mg myc-ARG1-HA Whole Cell Lysate. -4 µL anti-MYC mouse monoclonal antibody and 60 μg of Immunomagnetic beads Protein A/G. -Primary antibody: -Anti-MYC mouse monoclonal antibody, at 1:100 dilution. -Secondary antibody: -Rabbit Anti-Mouse IgG F(ab)2/HRP at 1/10000 dilution. -Developed using the ECL technique. -Performed under reducing conditions. -Predicted band size: 40 kDa. -Observed band size:40 kDa |
| 应用 | WB,IP |
| 推荐剂量 | WB: 1:5000-1:10000; IP: 1-5 µL/mg of lysate |
| 抗体种类 | Monoclonal |
| 宿主来源 | Mouse |
| 构建方式 | This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, a synthetic Myc tag peptide conjugated to KLH. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. |
| 纯化方式 | Protein A |
| 性状 | Liquid |
| 缓冲液 | 0.2 μm filtered solution in PBS |
| 研究背景 | The Myc tag is a popular protein tag derived from c-myc gene product with a molecular weight of 1.2 KDa. The amino acid sequence is EQKLISEEDL, corresponding to the C-terminal amino acids (410-419) of human c-myc protein. Anti-Myc Tag antibodies can facilitate the detection, separation and purification of Myc tag target proteins without protein-specific antibodies or probes in several immunochemical assays such as Western Blotting, ELISAs and immunostaining techniques. |
| 偶联 | Unconjugated |
| 免疫原 | A synthetic Myc tag peptide conjugated to KLH |
| 抗原种属 | other |
存储&运输
| 储存方式 | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| 运输方式 | Shipping with blue ice. |
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比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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