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Anti-Myc Tag Antibody (2E237) 是一种 Mouse 抗体,靶向 Myc Tag。Anti-Myc Tag Antibody (2E237) 可用于 IP。
Anti-Myc Tag Antibody (2E237) 是一种 Mouse 抗体,靶向 Myc Tag。Anti-Myc Tag Antibody (2E237) 可用于 IP。
规格 | 价格 | 库存 | 数量 |
---|---|---|---|
20 μL | ¥ 795 | 5日内发货 | |
100 μL | ¥ 1,585 | 5日内发货 | |
500 μL | ¥ 3,980 | 5日内发货 |
产品描述 | Anti-Myc Tag Antibody (2E237) is a Mouse antibody targeting Myc Tag. Anti-Myc Tag Antibody (2E237) can be used in IP. |
Ig Type | Monoclonal Mouse IgG1 |
克隆号 | 2E237 |
交叉反应 | other |
验证活性 | MYC tag was immunoprecipitated using: -Lane A:0.5 mg myc-ARG1-HA transfected 293 Whole Cell Lysate. -Lane B:0.5 mg HA-ARG1-myc transfected 293 Whole Cell Lysate. -2 µL anti-MYC tag mouse monoclonal antibody and 15 μl of 50 % Protein G agarose. -Primary antibody: -Anti-MYC tag mouse monoclonal antibody, at 1:100 dilution. -Secondary antibody: -Dylight 800-labeled antibody to Mouse IgG (H+L), at 1:7500 dilution. -Developed using the odyssey technique. -Performed under reducing conditions. -Predicted band size: 39 kDa. -Observed band size: 39 kDa |
应用 | IP |
推荐剂量 | IP: 1-4 μg/mg of lysate |
抗体种类 | Monoclonal |
宿主来源 | Mouse |
构建方式 | This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, a synthetic Myc tag peptide conjugated to KLH. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. |
纯化方式 | Protein A |
性状 | Liquid |
缓冲液 | 0.2 μm filtered solution in PBS |
研究背景 | The Myc tag is a popular protein tag derived from c-myc gene product with a molecular weight of 1.2 KDa. The amino acid sequence is EQKLISEEDL, corresponding to the C-terminal amino acids (410-419) of human c-myc protein. Anti-Myc Tag antibodies can facilitate the detection, separation and purification of Myc tag target proteins without protein-specific antibodies or probes in several immunochemical assays such as Western Blotting, ELISAs and immunostaining techniques. |
偶联 | Unconjugated |
其他可选修饰符 | HRP |
可提供抗体种类 | 2 |
免疫原 | A synthetic Myc tag peptide conjugated to KLH |
抗原种属 | other |
储存方式 | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
运输方式 | Shipping with blue ice. |
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