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Anti-Myc Tag Antibody (2E237)

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产品编号 TMAY-00031

Anti-Myc Tag Antibody (2E237) 是一种 Mouse 抗体，靶向 Myc Tag。Anti-Myc Tag Antibody (2E237) 可用于 IP。

Anti-Myc Tag Antibody (2E237)

Anti-Myc Tag Antibody (2E237)

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Rating icon 还可以
产品编号 TMAY-00031

Anti-Myc Tag Antibody (2E237) 是一种 Mouse 抗体，靶向 Myc Tag。Anti-Myc Tag Antibody (2E237) 可用于 IP。

规格价格库存数量
20 μL
¥ 795
5日内发货
100 μL
¥ 1,585
5日内发货
500 μL
¥ 3,980
5日内发货
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产品介绍

生物活性
产品描述
Anti-Myc Tag Antibody (2E237) is a Mouse antibody targeting Myc Tag. Anti-Myc Tag Antibody (2E237) can be used in IP.
Ig Type
Mouse IgG1
克隆号
2E237
交叉反应
other
特异性
Recognize N-terminal, C-terminal in fusion proteins.
验证活性
MYC tag was immunoprecipitated using:
-Lane A:0.5 mg myc-ARG1-HA transfected 293 Whole Cell Lysate.
-Lane B:0.5 mg HA-ARG1-myc transfected 293 Whole Cell Lysate.
-2 µL anti-MYC tag mouse monoclonal antibody and 15 μl of 50 % Protein G agarose.
-Primary antibody:
-Anti-MYC tag mouse monoclonal antibody, at 1:100 dilution.
-Secondary antibody:
-Dylight 800-labeled antibody to Mouse IgG (H+L), at 1:7500 dilution.
-Developed using the odyssey technique.
-Performed under reducing conditions.
-Predicted band size: 39 kDa.
-Observed band size: 39 kDa
应用IP
推荐剂量
IP: 1-4 μg/mg of lysate
抗体种类
Monoclonal
宿主来源Mouse
构建方式This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, a synthetic Myc tag peptide conjugated to KLH. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
纯化方式Protein A
性状Liquid
缓冲液0.2 μm filtered solution in PBS
研究背景The Myc tag is a popular protein tag derived from c-myc gene product with a molecular weight of 1.2 KDa. The amino acid sequence is EQKLISEEDL, corresponding to the C-terminal amino acids (410-419) of human c-myc protein. Anti-Myc Tag antibodies can facilitate the detection, separation and purification of Myc tag target proteins without protein-specific antibodies or probes in several immunochemical assays such as Western Blotting, ELISAs and immunostaining techniques.
偶联与修饰
偶联
Unconjugated
其他可选修饰符
HRP
可提供抗体种类
2
抗原信息
免疫原
A synthetic peptide: Myc tag peptide conjugated to KLH
抗原种属
other
存储&运输
储存方式Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free.
运输方式Shipping with blue ice.

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