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Anti-MXRA8 Polyclonal Antibody 是一种 Rabbit 抗体,靶向 MXRA8。Anti-MXRA8 Polyclonal Antibody 可用于 WB,IHC-P,IHC-Fr,IF。
Anti-MXRA8 Polyclonal Antibody 是一种 Rabbit 抗体,靶向 MXRA8。Anti-MXRA8 Polyclonal Antibody 可用于 WB,IHC-P,IHC-Fr,IF。
规格 | 价格 | 库存 | 数量 |
---|---|---|---|
50 μL | ¥ 1,160 | 5日内发货 | |
100 μL | ¥ 1,965 | 5日内发货 | |
200 μL | ¥ 2,780 | 5日内发货 |
产品描述 | Anti-MXRA8 Polyclonal Antibody is a Rabbit antibody targeting MXRA8. Anti-MXRA8 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF. |
Ig Type | IgG |
交叉反应 | Human,Mouse,Rat (predicted:Dog,Pig,Cow,Sheep) |
验证活性 | 1. Sample: Ovary (Mouse) Lysate at 40 μg MCF-7 (Human) Cell Lysate at 30 μg MD-MB-231 (Human) Cell Lysate at 30 μg Primary: Anti-MXRA8 (TMAB-01188) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 47 kDa Observed band size: 47 kDa 2. Paraformaldehyde-fixed, paraffin embedded (rat cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (MXRA8) Polyclonal Antibody, Unconjugated (TMAB-01188) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 3. Paraformaldehyde-fixed, paraffin embedded (human cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (MXRA8) Polyclonal Antibody, Unconjugated (TMAB-01188) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 4. Paraformaldehyde-fixed, paraffin embedded (mouse cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (MXRA8) Polyclonal Antibody, Unconjugated (TMAB-01188) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 5. Sample: Lane 1: Human U-2 OS cell lysates Lane 2: Human HepG2 cell lysates Primary: Anti-MXRA8 (TMAB-01188) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 47 kDa Observed band size: 56 kDa |
应用 | |
推荐剂量 | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
抗体种类 | Polyclonal |
宿主来源 | Rabbit |
亚细胞定位 | Membrane. |
构建方式 | Hybridoma Polyclonal Antibody |
纯化方式 | Protein A purified |
性状 | Liquid |
缓冲液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
研究背景 | MXRA8 is a 442 amino acid single-pass type I membrane protein that is implicated in the maintenance and maturation of the blood-brain barrier. Containing two Ig-like V-type (immunoglobulin-like) domains, MXRA8 exists as two alternatively spliced isoforms that are encoded by a gene that maps to human chromosome 1p36.33. Human chromosome 1 spans 260 million base pairs, contains over 3,000 genes and comprises nearly 8% of the human genome. Chromosome 1 houses a large number of disease-associated genes, including those that are involved in familial adenomatous polyposis, Stickler syndrome, Parkinson’s disease, Gaucher disease, schizophrenia and Usher syndrome. Aberrations in chromosome 1 are found in a variety of cancers, including head and neck cancer, malignant melanoma and multiple myeloma |
免疫原 | KLH conjugated synthetic peptide: human MXRA8 |
抗原种属 | Human |
基因ID | |
Uniprot ID | |
研究领域 | Other Antibodies |
储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
运输方式 | Shipping with blue ice. |
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