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Anti-Mucin-2/MUC2 Antibody (1B747) 是一种 Rabbit 抗体,靶向 Mucin-2/MUC2。Anti-Mucin-2/MUC2 Antibody (1B747) 可用于 WB,IHC-P,IHC-Fr,IF,FCM。
Anti-Mucin-2/MUC2 Antibody (1B747) 是一种 Rabbit 抗体,靶向 Mucin-2/MUC2。Anti-Mucin-2/MUC2 Antibody (1B747) 可用于 WB,IHC-P,IHC-Fr,IF,FCM。
规格 | 价格 | 库存 | 数量 |
---|---|---|---|
25 μL | ¥ 790 | 5日内发货 | |
50 μL | ¥ 1,390 | 5日内发货 | |
100 μL | ¥ 2,480 | 5日内发货 |
产品描述 | Anti-Mucin-2/MUC2 Antibody (1B747) is a Rabbit antibody targeting Mucin-2/MUC2. Anti-Mucin-2/MUC2 Antibody (1B747) can be used in WB,IHC-P,IHC-Fr,IF,FCM. |
Ig Type | IgG |
克隆号 | 1B747 |
交叉反应 | Human,Rat |
验证活性 | 1. Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (MUC2) Monoclonal Antibody, Unconjugated (TMAB-01186) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 2. Paraformaldehyde-fixed, paraffin embedded (human colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (MUC2) Monoclonal Antibody, Unconjugated (TMAB-01186) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 3. Paraformaldehyde-fixed, paraffin embedded (Human duodenum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (MUC2) Monoclonal Antibody, Unconjugated (TMAB-01186) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 4. Paraformaldehyde-fixed, paraffin embedded Rat Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MUC2 Monoclonal Antibody, Unconjugated (TMAB-01186) at 1:100 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. 5. 25 μg total protein per Lane of various lysates probed with MUC2 monoclonal antibody, unconjugated (TMAB-01186) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at RT for 60 min. |
应用 | |
推荐剂量 | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; FCM: 1:50-100 |
抗体种类 | Monoclonal |
宿主来源 | Rabbit |
亚细胞定位 | Secreted. Note=In the intestine, secreted into the inner and outer mucus layers. |
构建方式 | Recombinant Antibody |
纯化方式 | Protein A purified |
性状 | Liquid |
缓冲液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
研究背景 | This gene encodes a member of the mucin protein family. Mucins are high molecular weight glycoproteins produced by many epithelial tissues. The protein encoded by this gene is secreted and forms an insoluble mucous barrier that protects the gut lumen. The protein polymerizes into a gel of which 80% is composed of oligosaccharide side chains by weight. The protein features a central domain containing tandem repeats rich in threonine and proline that varies between 50 and 115 copies in different individuals. Alternatively spliced transcript variants of this gene have been described, but their full-length nature is not known. [provided by RefSeq, Jul 2008] |
免疫原 | KLH conjugated synthetic peptide: human MUC2 |
抗原种属 | Human |
基因ID | |
Uniprot ID | |
研究领域 | MUC,Tumor-associated antigens,MUC,Tumor Associated |
储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
运输方式 | Shipping with blue ice. |
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