Anti-Mucin-16/MUC16 Antibody (8O689) is a Mouse antibody targeting Mucin-16/MUC16. Anti-Mucin-16/MUC16 Antibody (8O689) can be used in WB,ICC/IF,FCM,IHC-P,IHC-Fr,IF.

IgG

8O689

Human

1. Paraformaldehyde-fixed, paraffin embedded Human Ovarian mucinous Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MUC16 Monoclonal Antibody, Unconjugated (TMAB-01185) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
2. (Negative control) paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MUC16 Monoclonal Antibody, Unconjugated (TMAB-01185) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded Human Uterus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MUC16 Monoclonal Antibody, Unconjugated (TMAB-01185) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
4. Paraformaldehyde-fixed, paraffin embedded Human Endometrial Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MUC16 Monoclonal Antibody, Unconjugated (TMAB-01185) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
5. Paraformaldehyde-fixed, paraffin embedded Human Endometrial; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MUC16 Monoclonal Antibody, Unconjugated (TMAB-01185) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
6. 25 μg total protein per Lane of various lysates probed with MUC16 monoclonal antibody, unconjugated (TMAB-01185) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at RT for 60 min.
7. 4% Paraformaldehyde-fixed OVCAR-3 (H) cell; Triton X-100 at RT for 20 min; Antibody incubation with (MUC16) monoclonal Antibody, unconjugated (TMAB-01185) 1:100, 90 min at 37°C; followed by conjugated Goat Anti-Mouse IgG antibody (green) at 37°C for 90 min, DAPI (blue) was used to stain the cell nucleus. PBS instead of the primary antibody was used as the blank control.
8. The OVCAR-3 (H) cells were incubated in 5% BSA to block non-specific protein-protein interactions (30 min at room temperature). Primary Antibody (green): Mouse Anti-MUC16 antibody (TMAB-01185): 1 μg/10^6 cells; Secondary Antibody (white blue): Goat anti-Mouse IgG-BF488: 1 μg/test. Isotype Control (orange): Mouse IgG. Blank control (black): PBS.

WB: 1:500-2000; ICC/IF: 1:50-200; FCM: 1μg/Test; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Monoclonal

Recombinant Protein: human MUC16 Protein

Human

MUC,Tumor biomarkers,Tumor antigens,MUC