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Anti-MTLC Polyclonal Antibody 是一种 Rabbit 抗体,靶向 MTLC。Anti-MTLC Polyclonal Antibody 可用于 WB,IHC-P,IHC-Fr,IF。
Anti-MTLC Polyclonal Antibody 是一种 Rabbit 抗体,靶向 MTLC。Anti-MTLC Polyclonal Antibody 可用于 WB,IHC-P,IHC-Fr,IF。
规格 | 价格 | 库存 | 数量 |
---|---|---|---|
50 μL | ¥ 1,170 | 5日内发货 | |
100 μL | ¥ 1,975 | 5日内发货 | |
200 μL | ¥ 2,790 | 5日内发货 |
产品描述 | Anti-MTLC Polyclonal Antibody is a Rabbit antibody targeting MTLC. Anti-MTLC Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF. |
Ig Type | IgG |
交叉反应 | Human,Mouse (predicted:Rat,Dog) |
验证活性 | 1. Sample: Brain (Mouse) Lysate at 40 μg Primary: Anti-MTLC (TMAB-01175) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/10000 dilution Predicted band size: 27 kDa Observed band size: 31 kDa 2. Tissue/cell: human colon carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Incubation: Anti-MTLC Polyclonal Antibody, Unconjugated (TMAB-01175) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining. |
应用 | |
推荐剂量 | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
抗体种类 | Polyclonal |
宿主来源 | Rabbit |
亚细胞定位 | Nucleus. Note=Expressed in nuclei of hepatocellular carcinoma cell line Bel7402 cells. |
构建方式 | Hybridoma Polyclonal Antibody |
纯化方式 | Protein A purified |
性状 | Liquid |
缓冲液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
研究背景 | MTLC is a 235 amino acid nuclear protein that is thought to regulate certain myc target genes. Downregulated in gastric cancer tissues, MTLC is expressed in the hepatocellular carcinoma cell line, Bel7402. Overexpression of MTLC is thought to influence tumorigenic conversion, promotion of apoptosis genomic instability and inhibition of hematopoietic differentiation. The gene encoding MTLC maps to human chromosome 6, which contains 170 million base pairs and comprises nearly 6% of the human genome. Deletion of a portion of the q arm of chromosome 6 is associated with early onset intestinal cancer, suggesting the presence of a cancer susceptibility locus. Additionally, Porphyria cutanea tarda, Parkinson's disease, Stickler syndrome and a susceptibility to bipolar disorder are all associated with genes that map to chromosome 6. |
免疫原 | KLH conjugated synthetic peptide: human MTLC |
抗原种属 | Human |
基因ID | |
Uniprot ID | |
研究领域 | Transcription Factors |
储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
运输方式 | Shipping with blue ice. |
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