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顶部产品介绍抗原信息化学信息储存和溶解信息计算器

Anti-MPND Polyclonal Antibody

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货号 TMAB-08963

Anti-MPND Polyclonal Antibody 是一种 Rabbit 抗体，靶向 MPND。Anti-MPND Polyclonal Antibody 可用于 WB,IHC-P,IHC-Fr,IF。

Anti-MPND Polyclonal Antibody

Anti-MPND Polyclonal Antibody

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Rating icon 还可以
货号 TMAB-08963

Anti-MPND Polyclonal Antibody 是一种 Rabbit 抗体，靶向 MPND。Anti-MPND Polyclonal Antibody 可用于 WB,IHC-P,IHC-Fr,IF。

规格价格库存数量
50 μL
¥ 1,160
5日内发货
100 μL
¥ 1,975
5日内发货
200 μL
¥ 2,785
5日内发货
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产品介绍

生物活性
产品描述
Anti-MPND Polyclonal Antibody is a Rabbit antibody targeting MPND. Anti-MPND Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Ig Type
IgG
反应种属
Human,Mouse,Rat (predicted:Dog,Pig,Cow,Sheep)
验证活性
1. Sample: SH-SY5Y (Human) Cell Lysate at 30 μg
Primary: Anti-MPND (TMAB-08963) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 50 kD
Observed band size: 52 kD
2. Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (MPND) Polyclonal Antibody, Unconjugated (TMAB-08963) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded (mouse colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (MPND) Polyclonal Antibody, Unconjugated (TMAB-08963) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.
4. Paraformaldehyde-fixed, paraffin embedded (mouse spleen); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (MPND) Polyclonal Antibody, Unconjugated (TMAB-08963) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.
5. 25 μg total protein per lane of various lysates (see on figure) probed with MPND polyclonal antibody, unconjugated (TMAB-08963) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.
应用WBIHC-PIHC-FrIF
推荐剂量
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500
抗体种类
Polyclonal
宿主来源Rabbit
构建方式Polyclonal Antibody
纯化方式Protein A purified
性状Liquid
缓冲液0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
浓度1mg/ml
研究背景Proteases are enzymes that are involved in protein catabolism by hydrolyzing the peptide bonds between amino acids in a polypeptide chain. MPND (MPN domain-containing protein) is a 471 amino acid protein that is thought to be a protease. Expressed as two isoforms produced by alternative splicing, MPND contains a JAMM motif and one MPN domain. The gene that encodes MPND maps to human chromosome 19, which consists of around 63 million bases with over 1,400 genes, making up over 2% of human genomic DNA. Chromosome 19 includes a diversity of interesting genes and is recognized for having the greatest gene density of the human chromosomes. It is the genetic home for a number of immunoglobulin superfamily members including the killer cell and leukocyte Ig-like receptors, a number of ICAMs, the CEACAM and PSG family and Fcα receptors. Key genes for eye color and hair color also map to chromosome 19.
抗原信息
免疫原
KLH conjugated synthetic peptide: human MPND
抗原种属
Human
基因名称
Mpnd
基因ID
68047
蛋白名称
MPN domain-containing protein
功能
Probable protease.
化学信息
分子量Theoretical: 50 kDa.
储存&溶解度
储存方式Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
运输方式Shipping with blue ice.

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