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Anti-MKI67 Polyclonal Antibody 2
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产品编号 TMAB-01145
Anti-MKI67 Polyclonal Antibody 2 是一种 Rabbit 抗体，靶向 MKI67。Anti-MKI67 Polyclonal Antibody 2 可用于 IHC-P,IHC-Fr,ICC/IF,IF。
Anti-MKI67 Polyclonal Antibody 2
Anti-MKI67 Polyclonal Antibody 2
还可以产品编号 TMAB-01145
Anti-MKI67 Polyclonal Antibody 2 是一种 Rabbit 抗体，靶向 MKI67。Anti-MKI67 Polyclonal Antibody 2 可用于 IHC-P,IHC-Fr,ICC/IF,IF。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 50 μL | ¥ 1,170 | 5日内发货 | |
| 100 μL | ¥ 1,965 | 5日内发货 | |
| 200 μL | ¥ 2,795 | 5日内发货 |
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产品介绍
生物活性
抗原信息
| 产品描述 | Anti-MKI67 Polyclonal Antibody 2 is a Rabbit antibody targeting MKI67. Anti-MKI67 Polyclonal Antibody 2 can be used in IHC-P,IHC-Fr,ICC/IF,IF. |
| Ig Type | IgG |
| 交叉反应 | Human |
| 验证活性 | 1. Paraformaldehyde-fixed, paraffin embedded Human Lung Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Ki-67 Polyclonal Antibody, Unconjugated (TMAB-01145) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining. 2. Paraformaldehyde-fixed, paraffin embedded Human Stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Ki-67 Polyclonal Antibody, Unconjugated (TMAB-01145) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining. 3. 4% Paraformaldehyde-fixed Hela (H) cell; Triton X-100 at RT for 20 min; Antibody incubation with (Ki-67) polyclonal Antibody, unconjugated (TMAB-01145) 1:100, 90 min at 37°C; followed by conjugated Goat Anti-Rabbit IgG antibody (green) at 37°C for 90 min, DAPI (blue) was used to stain the cell nucleus. PBS instead of the primary antibody was used as the blank control. |
| 应用 | |
| 推荐剂量 | IHC-P: 1:200-800; IHC-Fr: 1:200-800; ICC/IF: 1:100; IF: 1:300-800 |
| 抗体种类 | Polyclonal |
| 宿主来源 | Rabbit |
| 亚细胞定位 | Nucleus. Chromosome. Predominantly localized in the G1 phase in the perinucleolar region, in the later phases it is also detected throughout the nuclear interior, being predominantly localized in the nuclear matrix. In mitosis, it is present on all chromosomes. |
| 构建方式 | Hybridoma Polyclonal Antibody |
| 纯化方式 | Protein A purified |
| 性状 | Liquid |
| 缓冲液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究背景 | Ki67 antigen is the prototypic cell cycle related nuclear protein, expressed by proliferating cells in all phases of the active cell cycle (G1, S, G2 and M phase). It is absent in resting (G0) cells. Ki67 antibodies are useful in establishing the cell growing fraction in neoplasms (immunohistochemically quantified by determining the number of Ki67 positive cells among the total number of resting cells = Ki67 index). In neoplastic tissues the prognostic value is comparable to the tritiated thymidine labelling index. The correlation between low Ki67 index and histologically low grade tumours is strong. Ki67 is routinely used as a neuronal marker of cell cycling and proliferation. |
| 免疫原 | KLH conjugated synthetic peptide: human Ki-67 |
| 抗原种属 | Human |
| 基因ID | |
| Uniprot ID | |
| 研究领域 | Cell division,Tumor biomarkers,Markers,Soma marker,Neurogenesis,Replication |
存储&运输
| 储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |
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比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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