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顶部产品介绍抗原信息化学信息储存和溶解信息计算器

Anti-MKI67 Polyclonal Antibody

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货号 TMAB-01144

别名 Proliferation marker protein Ki-67, MKI67, Antigen identified by monoclonal antibody Ki-67 (Antigen KI-67;Antigen Ki67)

Anti-MKI67 Polyclonal Antibody 是一种 Rabbit 抗体，靶向 MKI67。Anti-MKI67 Polyclonal Antibody 可用于 ICC/IF,IF,IHC-Fr,IHC-P。

Anti-MKI67 Polyclonal Antibody

Anti-MKI67 Polyclonal Antibody

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货号 TMAB-01144 别名 Proliferation marker protein Ki-67, MKI67, Antigen identified by monoclonal antibody Ki-67 (Antigen KI-67;Antigen Ki67)

Anti-MKI67 Polyclonal Antibody 是一种 Rabbit 抗体，靶向 MKI67。Anti-MKI67 Polyclonal Antibody 可用于 ICC/IF,IF,IHC-Fr,IHC-P。

规格价格库存数量
50 μL
¥ 1,170
5日内发货
100 μL
¥ 1,960
5日内发货
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产品介绍

生物活性
产品描述
Anti-MKI67 Polyclonal Antibody is a Rabbit antibody targeting MKI67. Anti-MKI67 Polyclonal Antibody can be used in ICC/IF,IF,IHC-Fr,IHC-P.
别名Proliferation marker protein Ki-67, MKI67, Antigen identified by monoclonal antibody Ki-67 (Antigen KI-67;Antigen Ki67)
Ig Type
IgG
反应种属
Human
验证活性
1. 4% Paraformaldehyde-fixed Hela (H) cell; Triton X-100 at RT for 20 min; Antibody incubation with (Ki-67) polyclonal Antibody, unconjugated (TMAB-01144) 1:100, 90 min at 37°C; followed by conjugated Goat Anti-Rabbit IgG antibody (green) at 37°C for 90 min, DAPI (blue) was used to stain the cell nucleus. PBS instead of the primary antibody was used as the blank control.
2. Paraformaldehyde-fixed, paraffin embedded (human lung carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Ki-67) Polyclonal Antibody, Unconjugated (TMAB-01144) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded (Human stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Ki-67) Polyclonal Antibody, Unconjugated (TMAB-01144) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
4. Paraformaldehyde-fixed, paraffin embedded Human ovarian serous carcinoma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Ki-67 Polyclonal Antibody, Unconjugated (TMAB-01144) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
5. Paraformaldehyde-fixed, paraffin embedded Human Tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Ki-67 Polyclonal Antibody, Unconjugated (TMAB-01144) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
verifiedActivityverifiedActivityverifiedActivityverifiedActivityverifiedActivity
应用ICC/IFIFIHC-FrIHC-P
推荐剂量
IHC-P: 1:200-800; IHC-Fr: 1:200-800; ICC/IF: 1:100; IF: 1:300-800
抗体种类
Polyclonal
宿主来源Rabbit
亚细胞定位Nucleus. Chromosome. Predominantly localized in the G1 phase in the perinucleolar region, in the later phases it is also detected throughout the nuclear interior, being predominantly localized in the nuclear matrix. In mitosis, it is present on all chromosomes.
构建方式Polyclonal Antibody
纯化方式Protein A purified
性状Liquid
缓冲液0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
浓度1 mg/mL
研究背景Ki67 antigen is the prototypic cell cycle related nuclear protein, expressed by proliferating cells in all phases of the active cell cycle (G1, S, G2 and M phase). It is absent in resting (G0) cells. Ki67 antibodies are useful in establishing the cell growing fraction in neoplasms (immunohistochemically quantified by determining the number of Ki67 positive cells among the total number of resting cells = Ki67 index). In neoplastic tissues the prognostic value is comparable to the tritiated thymidine labelling index. The correlation between low Ki67 index and histologically low grade tumours is strong. Ki67 is routinely used as a neuronal marker of cell cycling and proliferation.
抗原信息
免疫原
KLH conjugated synthetic peptide: human Ki67
抗原种属
Human
基因名称
MKI67
基因ID
4288
蛋白名称
Proliferation marker protein Ki-67
Uniprot ID
P46013
研究领域
Cell division,Tumor biomarkers,Markers,Soma marker,Neurogenesis,Replication
功能
Thought to be required for maintaining cell proliferation.
化学信息
分子量Theoretical: 358 kDa. Actual: 320/359? kDa.
储存&溶解度
储存方式Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
运输方式Shipping with blue ice.
存储store at low temperature | store at -20°C

计算器

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关键词

MKI 67Antigen Ki-67Antigen Ki67
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