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顶部产品介绍抗原信息化学信息储存和溶解信息计算器

Anti-MKI67 Antibody (3A821)

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货号 TMAB-01141

别名 Proliferation marker protein Ki-67, MKI67, Antigen identified by monoclonal antibody Ki-67 (Antigen KI-67;Antigen Ki67)

Anti-MKI67 Antibody (3A821) 是一种 Rabbit 抗体，靶向 MKI67。Anti-MKI67 Antibody (3A821) 可用于 ICC/IF,IF,IHC-Fr,IHC-P。

Anti-MKI67 Antibody (3A821)

Anti-MKI67 Antibody (3A821)

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Rating icon 还可以
货号 TMAB-01141 别名 Proliferation marker protein Ki-67, MKI67, Antigen identified by monoclonal antibody Ki-67 (Antigen KI-67;Antigen Ki67)

Anti-MKI67 Antibody (3A821) 是一种 Rabbit 抗体，靶向 MKI67。Anti-MKI67 Antibody (3A821) 可用于 ICC/IF,IF,IHC-Fr,IHC-P。

规格价格库存数量
25 μL
¥ 795
5日内发货
50 μL
¥ 1,385
5日内发货
100 μL
¥ 2,480
5日内发货
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产品介绍

生物活性
产品描述
Anti-MKI67 Antibody (3A821) is a Rabbit antibody targeting MKI67. Anti-MKI67 Antibody (3A821) can be used in ICC/IF,IF,IHC-Fr,IHC-P.
别名Proliferation marker protein Ki-67, MKI67, Antigen identified by monoclonal antibody Ki-67 (Antigen KI-67;Antigen Ki67)
Ig Type
IgG
克隆号
3A821
反应种属
Human,Mouse,Rat
验证活性
1. Paraformaldehyde-fixed, paraffin embedded Mouse Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Ki67Monoclonal Antibody, Unconjugated (TMAB-01141) at 1:500 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
PBS instead of the primary antibody was used as the blank control.
2. Paraformaldehyde-fixed, paraffin embedded Mouse Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Ki67Monoclonal Antibody, Unconjugated (TMAB-01141) at 1:500 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded Rat Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Ki67Monoclonal Antibody, Unconjugated (TMAB-01141) at 1:500 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
4. Paraformaldehyde-fixed, paraffin embedded Rat Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Ki67Monoclonal Antibody, Unconjugated (TMAB-01141) at 1:500 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
5. Paraformaldehyde-fixed, paraffin embedded Rat Skin; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Ki67Monoclonal Antibody, Unconjugated (TMAB-01141) at 1:500 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
6. Paraformaldehyde-fixed, paraffin embedded Rat Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Ki67Monoclonal Antibody, Unconjugated (TMAB-01141) at 1:500 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
7. Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Ki67Monoclonal Antibody, Unconjugated (TMAB-01141) at 1:500 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
8. Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Ki67Monoclonal Antibody, Unconjugated (TMAB-01141) at 1:500 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
9. Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Ki67Monoclonal Antibody, Unconjugated (TMAB-01141) at 1:500 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
10. Paraformaldehyde-fixed, paraffin embedded Human Ovarian Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Ki67Monoclonal Antibody, Unconjugated (TMAB-01141) at 1:500 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
11. Paraformaldehyde-fixed, paraffin embedded Human Tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Ki67Monoclonal Antibody, Unconjugated (TMAB-01141) at 1:500 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
12. Paraformaldehyde-fixed, paraffin embedded Human Gastric Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Ki67Monoclonal Antibody, Unconjugated (TMAB-01141) at 1:500 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
13. (Negative control) Paraformaldehyde-fixed, paraffin embedded Rat Heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Ki67 Monoclonal Antibody, Unconjugated (TMAB-01141) at 1:500 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
14. (Negative control) Paraformaldehyde-fixed, paraffin embedded Mouse Heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Ki67 Monoclonal Antibody, Unconjugated (TMAB-01141) at 1:500 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
15. 4% Paraformaldehyde-fixed Hela (H) cell; Triton X-100 at RT for 20 min; Antibody incubation with (Ki-67) monoclonal Antibody, unconjugated (TMAB-01141) 1:200, 90 min at 37°C; followed by conjugated Goat Anti-Rabbit IgG antibody (green) at 37°C for 90 min, DAPI (blue) was used to stain the cell nucleus. PBS instead of the primary antibody was used as the blank control.
16. Paraformaldehyde-fixed, paraffin embedded Rat Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Ki67 Monoclonal Antibody, Unconjugated (TMAB-01141) at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (green), DAPI (blue) was used to stain the cell nucleus.
17. Paraformaldehyde-fixed, paraffin embedded Human Esophageal Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Ki67 Monoclonal Antibody, Unconjugated (TMAB-01141) at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (green), DAPI (blue) was used to stain the cell nucleus.
18. Paraformaldehyde-fixed, paraffin embedded Rat Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Ki67 Polyclonal Antibody, Unconjugated (TMAB-01141) at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (green), DAPI (blue) was used to stain the cell nucleus.
verifiedActivityverifiedActivityverifiedActivityverifiedActivityverifiedActivityverifiedActivityverifiedActivityverifiedActivityverifiedActivityverifiedActivityverifiedActivityverifiedActivityverifiedActivityverifiedActivityverifiedActivityverifiedActivityverifiedActivityverifiedActivity
应用ICC/IFIFIHC-FrIHC-P
推荐剂量
IHC-P: 1:500-1000; IHC-Fr: 1:500-1000; ICC/IF: 1:200-500; IF: 1:300-1000
抗体种类
Monoclonal
宿主来源Rabbit
亚细胞定位Nucleus. Chromosome. Predominantly localized in the G1 phase in the perinucleolar region, in the later phases it is also detected throughout the nuclear interior, being predominantly localized in the nuclear matrix. In mitosis, it is present on all chromosomes.
构建方式Recombinant Antibody
纯化方式Protein A purified
性状Liquid
缓冲液0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
浓度1 mg/mL
研究背景Ki67 antigen is the prototypic cell cycle related nuclear protein, expressed by proliferating cells in all phases of the active cell cycle (G1, S, G2 and M phase). It is absent in resting (G0) cells. Ki67 antibodies are useful in establishing the cell growing fraction in neoplasms (immunohistochemically quantified by determining the number of Ki67 positive cells among the total number of resting cells = Ki67 index). In neoplastic tissues the prognostic value is comparable to the tritiated thymidine labelling index. The correlation between low Ki67 index and histologically low grade tumours is strong. Ki67 is routinely used as a neuronal marker of cell cycling and proliferation.
抗原信息
免疫原
KLH conjugated synthetic peptide: human Ki67
抗原种属
Human
基因名称
MKI67
基因ID
4288
蛋白名称
Proliferation marker protein Ki-67
Uniprot ID
P46013
研究领域
Cell division,Tumor biomarkers,Markers,Soma marker,Neurogenesis,Replication
功能
Thought to be required for maintaining cell proliferation.
化学信息
分子量Theoretical: 358 kDa.
储存&溶解度
储存方式Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
运输方式Shipping with blue ice.
存储store at low temperature | store at -20°C

计算器

  • 稀释 计算器

关键词

MKI 67Antigen Ki-67Antigen Ki67
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