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Anti-MHC Class II Polyclonal Antibody 2
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产品编号 TMAB-01135
Anti-MHC Class II Polyclonal Antibody 2 是一种 Rabbit 抗体，靶向 MHC Class II。Anti-MHC Class II Polyclonal Antibody 2 可用于 WB,IHC-P,IHC-Fr,IF。
Anti-MHC Class II Polyclonal Antibody 2
Anti-MHC Class II Polyclonal Antibody 2
还可以产品编号 TMAB-01135
Anti-MHC Class II Polyclonal Antibody 2 是一种 Rabbit 抗体，靶向 MHC Class II。Anti-MHC Class II Polyclonal Antibody 2 可用于 WB,IHC-P,IHC-Fr,IF。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 50 μL | ¥ 1,170 | 5日内发货 | |
| 100 μL | ¥ 1,975 | 5日内发货 | |
| 200 μL | ¥ 2,795 | 5日内发货 |
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产品介绍
生物活性
抗原信息
| 产品描述 | Anti-MHC Class II Polyclonal Antibody 2 is a Rabbit antibody targeting MHC Class II. Anti-MHC Class II Polyclonal Antibody 2 can be used in WB,IHC-P,IHC-Fr,IF. |
| Ig Type | IgG |
| 交叉反应 | Human,Mouse (predicted:Rat) |
| 验证活性 | 1. Protein: mouse lung lysates at 30 μg; Primary: Anti-MHC Class II (TMAB-01135) at 1:200; Secondary: HRP conjugated Goat Anti-Rabbit Igg (secondary antibody) at 1: 3000; ECL excitated the fluorescence; Predicted band size: 27 kDa Observed band size: 30 kDa 2. Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Incubation: Anti-MHC Class II Polyclonal Antibody, Unconjugated (TMAB-01135) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining. 3. Sample: Lane 1: Lung (Mouse) Lysate at 40 μg Lane 2: Cerebrum (Mouse) Lysate at 40 μg Lane 3: LympHnode (Mouse) Lysate at 40 μg Lane 4: Spleen (Mouse) Lysate at 40 μg Primary: Anti-MHC Class II (TMAB-01135) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 29/50 kDa Observed band size: 50 kDa |
| 应用 | |
| 推荐剂量 | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| 抗体种类 | Polyclonal |
| 宿主来源 | Rabbit |
| 亚细胞定位 | Cell membrane; Single-pass type II membrane protein (Potential). Endoplasmic reticulum membrane. Golgi apparatus, trans-Golgi network. Endosome. Lysosome. |
| 构建方式 | Hybridoma Polyclonal Antibody |
| 纯化方式 | Protein A purified |
| 性状 | Liquid |
| 缓冲液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究背景 | HLA-DMB belongs to the HLA class II beta chain paralogues. This class II molecule is a heterodimer consisting of an alpha (DMA) and a beta (DMB) chain, both anchored in the membrane. It is located in intracellular vesicles. DM plays a central role in the peptide loading of MHC class II molecules by helping to release the CLIP (class II-associated invariant chain peptide) molecule from the peptide binding site. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The beta chain is approximately 26-28 kDa and its gene contains 6 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, exon 4 encodes the transmembrane domain and exon 5 encodes the cytoplasmic tail. [provided by RefSeq, Jul 2008] |
| 免疫原 | KLH conjugated synthetic peptide: human HLA class II histocompatibility antigen DM beta chain |
| 抗原种属 | Human |
| 基因ID | |
| Uniprot ID | |
| 研究领域 | Class II |
存储&运输
| 储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |
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比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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