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Anti-MGMT Antibody (9E151)
还可以产品编号 TMAH-00755
别名 O-6-methylguanine-DNA-alkyltransferase, MGMT, Methylated-DNA--protein-cysteine methyltransferase, 6-O-methylguanine-DNAmethyltransferase
Anti-MGMT Antibody (9E151) 是一种抗体，靶向 MGMT。Anti-MGMT Antibody (9E151) 可用于 ELISA, WB, IHC。
Anti-MGMT Antibody (9E151)
Anti-MGMT Antibody (9E151)
还可以产品编号 TMAH-00755 别名 O-6-methylguanine-DNA-alkyltransferase, MGMT, Methylated-DNA--protein-cysteine methyltransferase, 6-O-methylguanine-DNAmethyltransferase
Anti-MGMT Antibody (9E151) 是一种抗体，靶向 MGMT。Anti-MGMT Antibody (9E151) 可用于 ELISA, WB, IHC。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 50 μL | ¥ 1,315 | 5日内发货 | |
| 100 μL | ¥ 2,195 | 5日内发货 |
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产品介绍
生物活性
偶联与修饰
抗原信息
| 产品描述 | Anti-MGMT Antibody (9E151) is an antibody targeting MGMT. Anti-MGMT Antibody (9E151) can be used in ELISA, WB, IHC. |
| 别名 | O-6-methylguanine-DNA-alkyltransferase, MGMT, Methylated-DNA--protein-cysteine methyltransferase, 6-O-methylguanine-DNAmethyltransferase |
| Ig Type | Rabbit IgG |
| 克隆号 | 9E151 |
| 交叉反应 | Human |
| 验证活性 | 1. Western Blot -Positive WB detected in: MCF-7 whole cell lysate, Jurkat whole cell lysate -All lanes: MGMT antibody at 1:1500 -Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution -Predicted band size: 22 kDa -Observed band size: 22 kDa 2. IHC image of TMAH-00755 diluted at 1:100 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB. |
| 应用 | ELISA, WB, IHC |
| 推荐剂量 | WB:1:500-1:5000; IHC:1:50-1:200. |
| 抗体种类 | Monoclonal |
| 亚细胞定位 | Nucleus. |
| 构建方式 | Recombinant Antibody |
| 纯化方式 | Affinity-chromatography |
| 性状 | Liquid |
| 缓冲液 | Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| 研究背景 | Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) and O4-methylthymine (O4-MeT) in DNA. Repairs the methylated nucleobase in DNA by stoichiometrically transferring the methyl group to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated. |
| 偶联 | Unconjugated |
| 免疫原 | A synthetic peptide: Human MGMT |
| 抗原种属 | Human |
| Uniprot ID | |
| 研究领域 | Epigenetics and Nuclear Signaling |
存储&运输
| 储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |
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比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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