Anti-MEK3 Polyclonal Antibody is a Rabbit antibody targeting MEK3. Anti-MEK3 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

IgG

Human,Mouse,Rat

1. Sample:
Muscle (Mouse) Lysate at 40 μg
Muscle (Mouse) Lysate at 40 μg
Primary: Anti-MEK3 (TMAB-08734) at 1/500 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 39 kD
Observed band size: 39 kD
2. Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (MEK3) Polyclonal Antibody, Unconjugated (TMAB-08734) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded (Rat stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (MEK3) Polyclonal Antibody, Unconjugated (TMAB-08734) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.
4. Formalin-fixed and paraffin embedded human skeletal muscle labeled with Rabbit Anti-MEK3/MAP2 k3 Polyclonal Antibody (TMAB-08734) at 1: 250 overnight at room temperature followed by conjugation to secondary antibody.
5. Sample: Skeletal muscle (Rat) Lysate at 40 μg
Primary: Anti-MEK3 (TMAB-08734) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 39 kD
Observed band size: 39 kD

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human MKK3

Human

MAP2K3

Dual specificity mitogen-activated protein kinase kinase 3

Dual specificity kinase. Is activated by cytokines and environmental stress in vivo. Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in the MAP kinase p38.