Anti-MCU Polyclonal Antibody is a Rabbit antibody targeting MCU. Anti-MCU Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF,ELISA.

IgG

Mouse,Rat (predicted:Human,Dog,Pig,Cow,Horse,Rabbit,Sheep)

Paraformaldehyde-fixed, paraffin embedded (rat testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (MCU) Polyclonal Antibody, Unconjugated (TMAB-01121) at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 min and DAB staining.

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; ICC/IF: 1:100-500; IF: 1:100-500; ELISA: 1:5000-10000

Polyclonal

KLH conjugated synthetic peptide: human MCU

Human

MCU

Calcium uniporter protein, mitochondrial

Cardiac metabolism,Mitochondrial markers

Mitochondrial inner membrane calcium uniporter that mediates calcium uptake into mitochondria. Mitochondrial calcium homeostasis plays key roles in cellular physiology and regulates cell bioenergetics, cytoplasmic calcium signals and activation of cell death pathways. Activity is regulated by MICU1 and MICU2 that stimulate and inhibit MCU activity, respectively. Regulates glucose-dependent insulin secretion in pancreatic beta-cells by regulating mitochondrial calcium uptake. Involved in buffering the amplitude of systolic calcium rises in cardiomyocytes.