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Anti-MBP tag Antibody (8B845)
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产品编号 TMAB-01111
Anti-MBP tag Antibody (8B845) 是一种 Mouse 抗体，靶向 MBP tag。Anti-MBP tag Antibody (8B845) 可用于 WB。
Anti-MBP tag Antibody (8B845)
Anti-MBP tag Antibody (8B845)
还可以产品编号 TMAB-01111
Anti-MBP tag Antibody (8B845) 是一种 Mouse 抗体，靶向 MBP tag。Anti-MBP tag Antibody (8B845) 可用于 WB。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 50 μL | ¥ 670 | 5日内发货 | |
| 100 μL | ¥ 970 | 5日内发货 | |
| 500 μL | ¥ 3,995 | 5日内发货 |
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产品介绍
生物活性
抗原信息
| 产品描述 | Anti-MBP tag Antibody (8B845) is a Mouse antibody targeting MBP tag. Anti-MBP tag Antibody (8B845) can be used in WB. |
| 克隆号 | 8B845 |
| 交叉反应 | Species independent |
| 验证活性 | 100 ng rhC-jun-His-MBP proteinper Lane probed with MBP tag monoclonal antibody respectively, unconjugated (TMAB-01111) at 1:10000 dilution and 4°C overnight incubation. Followed by corresponding conjugated secondary antibody incubation at RT for 60 min. |
| 应用 | |
| 推荐剂量 | WB: 1:1000-10000 |
| 抗体种类 | Monoclonal |
| 宿主来源 | Mouse |
| 亚细胞定位 | Periplasm. |
| 构建方式 | Hybridoma Monoclonal Antibody |
| 纯化方式 | Protein A purified |
| 性状 | Liquid |
| 缓冲液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究背景 | Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. Maltose binding protein(MBP) is the 370 amino acid product of the E.coli mal E gene. MBP is a useful affinity tag that can increase the expression level and solubility of the resulting tagged protein. The MBP tag also promotes proper folding of the attached protein. Plasmid vectors have been constructed utilizing the MBP domain that allow the synthesis of high levels of MBP-fusion proteins that can be purified in a one step procedure by affinity chromatography cross linked amylose resin. Once bound to amylose, the MBP protein can then be separated from the target protein by cleavage by coagulation Factor Xa at a specific four residue site. Alternatively, the intact fusion protein can be specifically eluted from the resin by the addition of excess free maltose. Subsequent to elution, MBP fusion protein can be visualized either by Western blot analysis or immunoprecipitation using antibodies specific for the MBP-tag. An antibody to MBP can also be used to isolate or detect expression of the protein. |
| 免疫原 | Recombinant Protein: MBP Protein of Escherichia coli (strain K12) |
存储&运输
| 储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |
计算器
比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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