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Anti-MBP Polyclonal Antibody

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Anti-MBP Polyclonal Antibody
产品编号 TMAB-01109

Anti-MBP Polyclonal Antibody 是一种 Rabbit 抗体,靶向 MBP。Anti-MBP Polyclonal Antibody 可用于 WB,IHC-P,IHC-Fr,IF。

Anti-MBP Polyclonal Antibody

Anti-MBP Polyclonal Antibody

Rating icon 还可以
Anti-MBP Polyclonal Antibody
产品编号 TMAB-01109

Anti-MBP Polyclonal Antibody 是一种 Rabbit 抗体,靶向 MBP。Anti-MBP Polyclonal Antibody 可用于 WB,IHC-P,IHC-Fr,IF。

规格价格库存数量
50 μL
¥ 1,175
5日内发货
100 μL
¥ 1,965
5日内发货
200 μL
¥ 2,780
5日内发货
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产品介绍

生物活性
产品描述
Anti-MBP Polyclonal Antibody is a Rabbit antibody targeting MBP. Anti-MBP Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Ig Type
IgG
交叉反应
Human,Mouse,Rat (predicted:Dog,Pig,Cow,Horse,Rabbit,Sheep)
验证活性
1. 25 μg total protein per Lane of various lysates probed with MBP polyclonal antibody, unconjugated (TMAB-01109) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at RT for 60 min.
2. Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MBP Polyclonal Antibody, Unconjugated (TMAB-01109) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded Mouse Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MBP Polyclonal Antibody, Unconjugated (TMAB-01109) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
4. Paraformaldehyde-fixed, paraffin embedded Rat Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MBP Polyclonal Antibody, Unconjugated (TMAB-01109) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
5. Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MBP Polyclonal Antibody, Unconjugated (TMAB-01109) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
6. Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MBP Polyclonal Antibody, Unconjugated (TMAB-01109) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
7. Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MBP Polyclonal Antibody, Unconjugated (TMAB-01109) at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (green), DAPI (blue) was used to stain the cell nucleus.
8. Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MBP Polyclonal Antibody, Unconjugated (TMAB-01109) at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (green), DAPI (blue) was used to stain the cell nucleus.
9. Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MBP Polyclonal Antibody, Unconjugated (TMAB-01109) at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (Rose red), DAPI (blue) was used to stain the cell nucleus.
10. Paraformaldehyde-fixed, paraffin embedded Mouse Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MBP Polyclonal Antibody, Unconjugated (TMAB-01109) at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (Rose red), DAPI (blue) was used to stain the cell nucleus.
11. Paraformaldehyde-fixed, paraffin embedded Rat Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MBP Polyclonal Antibody, Unconjugated (TMAB-01109) at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (Rose red), DAPI (blue) was used to stain the cell nucleus.
12. Paraformaldehyde-fixed, paraffin embedded Human Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MBP Polyclonal Antibody, Unconjugated (TMAB-01109) at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (Rose red), DAPI (blue) was used to stain the cell nucleus.
13. Paraformaldehyde-fixed, paraffin embedded Human Left Parietal Lobe; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MBP Polyclonal Antibody, Unconjugated (TMAB-01109) at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (Rose red), DAPI (blue) was used to stain the cell nucleus.
应用
推荐剂量
WB: 1:1000-5000; IHC-P: 1:500-2000; IHC-Fr: 1:500-2000; IF: 1:500-2000
抗体种类
Polyclonal
宿主来源Rabbit
亚细胞定位Myelin membrane; Peripheral membrane protein; Cytoplasmic side. Note=Cytoplasmic side of myelin.
构建方式Hybridoma Polyclonal Antibody
纯化方式Protein A purified
性状Liquid
缓冲液0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
研究背景Oligodendrocyte Marker The classic group of Myelin basic protein (MBP) isoforms (isoforms 4 to 14) are with PLP the most abundant protein components of the myelin membrane in the CNS. They have a role in both its formation and stabilization. The smaller isoforms might have an important role in remyelination of denuded axons in multiple sclerosis. The non classic group of MBP isoforms (isoforms 1 to 3/Golli MBPs) may preferentially have a role in the early developing brain long before myelination, maybe as components of transcriptional complexes, and may also be involved in signaling pathways in T cells and neural cells. Differential splicing events combined to optional posttranslational modifications give a wide spectrum of isomers, each of them having maybe a specialized function.
抗原信息
免疫原
KLH conjugated synthetic peptide: Gpig MBP
抗原种属
Pig
基因ID
Uniprot ID
研究领域
Membrane Proteins,Oligodendrocyte marker,Glial
存储&运输
储存方式Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
运输方式Shipping with blue ice.

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